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目的利用鼠骨肉瘤模型,观察各CT灌注参数、最大强化速率(MER)与肿瘤微血管密度(MVD)的关系;分析鼠骨肉瘤外周区与中央区各CT灌注参数、MER、MVD之间有无差异。方法Wistar大鼠30只,采用大鼠骨肉瘤细胞系UMR106,接种于大鼠股骨远端或胫骨近端的骨髓腔中。CT灌注检查:用CT自动高压注射器经大鼠尾静脉注射非离子型碘对比剂欧乃派克(300 mgI/ml),总量为1.5～2.0 ml,注射速度0.5 ml/s。电影采集,2 mm层厚,1次扫描4层,扫描速度1 s/周,扫描时间50 s,共得到200幅图像。CT灌注检查后,将鼠处死,取肿瘤标本行HE及免疫组织化学检查,免疫组织化学检查用SP法,用单克隆抗CD34抗体标记血管,用Weidner技术计算肿瘤MVD。结果肿瘤BF、BV的平均值分别为100.09 ml·min~(-1)·100 g~(-1)、10.81 ml·100 g~(-1),均高于正常肌肉组织(BF、BV平均值分别为60.45 ml·min~(-1)·100 g~(-1)、1.89 ml·100 g~(-1))。肿瘤的BF、BV和MER与肿瘤的MVD均呈正线性相关,即肿瘤的MVD越高,相应的BF、BV和MER也就越高。肿瘤外周区与中央区的BF、BV、MVD之间有显著性差异(P<0.05)。结论本研究显示鼠骨肉瘤的BF、BV均与MVD呈正线性相关,CT灌注成像可用于活体无创性地评价骨肿瘤的微血管生成,可以详细全面提供骨肿瘤医生制定手术计划所需要的骨肿瘤的性质和血供情况的信息。
OBJECTIVE: To observe the relationship between CT perfusion parameters, maximum rate of enhancement (MER) and tumor microvessel density (MVD) in murine osteosarcoma model. To analyze the CT perfusion parameters between the peripheral and central regions of murine osteosarcoma, MER and MVD difference. Methods Thirty Wistar rats were inoculated into the marrow cavity of distal or proximal tibia of rat using rat osteosarcoma cell line UMR106. CT perfusion examination: The non-ionic iodine contrast agent Omnipaque (300 mgI / ml) was injected through the tail vein of a rat with a CT autohypertensive syringe at a total volume of 1.5-2.0 ml at a rate of 0.5 ml / s. Film collection, 2 mm layer thickness, 1 scan 4 layers, scanning speed 1 s / week, scanning time 50 s, a total of 200 images. After CT perfusion examination, the mice were sacrificed and the tumor samples were taken for HE and immunohistochemistry. Immunohistochemical SP method was used to mark the blood vessels with monoclonal anti-CD34 antibody. Weigner technique was used to calculate the tumor MVD. Results The mean values of BF and BV were 100.09 ml · min -1 · 100 g -1 · 10.81 ml · 100 g -1, which were higher than those of normal muscle tissue The values were 60.45 ml · min -1 · 100 g -1, 1.89 ml · 100 g -1, respectively). The BF, BV and MER of the tumor were positively correlated with the MVD of the tumor, that is, the higher the MVD of the tumor, the higher the corresponding BF, BV and MER. There was a significant difference between BF, BV and MVD in the peripheral area and the central area (P <0.05). Conclusions This study shows that both BF and BV in murine osteosarcoma are positively correlated with MVD. CT perfusion imaging can be used to noninvasively evaluate the angiogenesis of bone tumor in vivo. It provides a comprehensive and comprehensive guideline for the oncology of bone tumors Nature and blood supply information.