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通过病理切片和HE染色技术,对自然发生体表溃烂病大鲵的皮肤、肌肉、肝、脾、肺、胃肠道、肾等器官的病理组织学变化进行了观察。结果显示,肠道黏膜层、黏膜下层及浆膜层有大量炎性细胞浸润,黏膜上皮细胞大量坏死;肝中央静脉扩张淤血,肝细胞肿胀,体积增大,部分肝细胞胞浆及胞核淡染、溶解、消失,只剩下细胞轮廓,肝间质充满大量淋巴细胞、单核细胞等炎性细胞;肾间质出血,大量红细胞浸润,肾小球毛细血管严重扩张、充血,肾小囊扩张,部分肾小囊内可见均质红染的蛋白样物质,肾小管上皮细胞空泡变性,部分肾小管上皮细胞坏死、溶解、消失,管内有大量管形出现;脾实质细胞弥漫性坏死;肺泡腔内充满数量不等的红细胞;肌肉可见间质水肿,间隙增宽,肌纤维零星分布;部分肌细胞肌浆凝固、红染,有的肌细胞胞浆溶解成蜂窝状,甚至胞浆大量消失,只剩下肌膜。表明,患病大鲵除体表皮肤肌肉变化外,肝、脾、肾等重要器官均出现不同程度的病理变化。
Histopathological changes of skin, muscle, liver, spleen, lung, gastrointestinal tract, kidney and other organs were observed by pathological sections and HE staining. The results showed that intestinal mucosa, submucosa and serosa have a large number of inflammatory cell infiltration, a large number of mucosal epithelial necrosis; central venous dilatation and congestion, hepatocyte swelling, increased volume, some of the hepatocyte cytoplasm and nucleus pale Dye, dissolved, disappeared, leaving only the outline of the cells, liver interstitial filled with a large number of lymphocytes, monocytes and other inflammatory cells; renal interstitial hemorrhage, a large number of erythrocyte infiltration, severe expansion of glomerular capillaries, congestion, Dilation, some of the renal cysts visible homogeneous red staining of protein-like substances, tubular epithelial cells vacuolar degeneration, some of renal tubular epithelial cells necrosis, dissolution, disappearance of a large number of tubular appearance; diffuse necrosis of splenic parenchymal cells; Alveolar cavity filled with a varying number of red blood cells; interstitial edema visible muscles, gap widened, sporadic distribution of muscle fibers; part of myocyte sarcoplasmic coagulation, red dye, and some muscle cell cytoplasm dissolved into a honeycomb, and even a large number of disappeared in the cytoplasm , Leaving only the muscle membrane. The results showed that in addition to body surface skin and muscle changes, the pathological changes of liver, spleen, kidney and other important organs were observed.