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利用中国北方稻区6个广致病谱稻瘟病菌系的混合菌液对我国和国外引进的334份野生稻材料进行注射接种鉴定,筛选出25份抗病或高度抗病的材料。鉴定结果发现对广致病谱菌系混合菌液表现抗病的材料,对日本7个代表菌系全部表现抗病,说明可以利用广致病谱稻瘟病菌系的混合接种源,广泛筛选抗源。本研究还利用日本7个代表菌系分别接种80份对6个广致病谱菌系的混合菌液感病的野生稻材料和日本12个具已知抗病基因的鉴别品种,根据反应型的比较分析,推断出41份野生稻材料的抗病基因。且通过常规杂交,已将高度抗病的野生稻抗病基因导入栽培稻品种中
In this study, 334 wild rice (Oryza sativa L.) introduced from China and abroad were inoculated with a mixture of 6 bacterial strains of the pathogenic fungus Magnaporthe grisea in northern China to identify 25 resistant or highly disease-resistant materials. Identification of the results showed that broad-spectrum strains of mixed bacteria showed disease resistance of the material, all of Japan’s seven representative strains of the disease resistance, indicating that you can use broad-spectrum pathogenic bacteria blast inoculation of mixed sources, extensive screening of anti- source. In this study, we also inoculated 80 strains of wild rice (Oryza sativa L.) which are susceptible to mixed bacteria in 6 broad-spectrum pathogenic bacteria strains and 12 discriminant varieties with known disease-resistance genes from 7 representative strains in Japan. Of the comparative analysis, inferred 41 wild rice material resistance genes. And the highly resistant wild rice resistance genes have been introduced into cultivated rice varieties by conventional hybridization