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AIM:To identify the role of herbal compound 861(Cpd 861)in the regulation of mRNA expression of collagen synthesis-and degradation-related genes in human hepatic stellate cells(HSCs). METHODS:mRNA levels of collagen typesⅠand III,matrix metalloproteinase 1(MMP-1),matrix metalloproteinase 2(MMP-2),membrane type-1 matrix metalloproteinase(MT1-MMP),tissue inhibitor of metalloproteinase 1(TIMP-1),and transforming growth factorβ1(TGF-β1)in cultured-activated HSCs treated with Cpd 861 or interferon-g(IFN-g)were determined by real-time PCR. RESULTS:Both Cpd 861 and IFN-g reduced the mRNA levels of collagen typeⅢ,MMP-2 and TGF-β1.Moreover, Cpd 861 significantly enhanced the MMP-1 mRNA levels while down-regulated the TIMP-1 mRNA expression, increasing the ratio of MMP-1 to TIMP-1 to(6.3+0.3)- fold compared to the control group. CONCLUSION:The anti-fibrosis function of Cpd 861 may be mediated by both decreased interstitial collagen sythesis by inhibiting the transcription of collagen type Ⅲand TGF-β1 and increased degradation of these collagens by up-regulating MMP-1 and down-regulating TIMP-1 mRNA levels.
AIM: To identify the role of herbal compound 861 (Cpd 861) in the regulation of mRNA expression of collagen synthesis-and degradation-related genes in human hepatic stellate cells (HSCs). METHODS: mRNA levels of collagen types I and III, matrix metalloproteinase 1 (MMP-2), membrane type-1 matrix metalloproteinase (MT1-MMP), tissue inhibitor of metalloproteinase 1 (TIMP-1), and transforming growth factor β1 (TGF- activated HSCs treated with Cpd 861 or interferon-g (IFN-g) were determined by real-time PCR. RESULTS: Both Cpd 861 and IFN-g reduced the mRNA levels of collagen type III, MMP- Cpd 861 significantly enhanced the MMP-1 mRNA expression, increasing the ratio of MMP-1 to TIMP-1 to (6.3 + 0.3) - fold compared to the control group. CONCLUSION: The anti -fibrosis function of Cpd 861 may be mediated by both decreased interstitial collagen sythesis by inhibiting the transcription of collagen type Ⅲ and TGF-β1 and increased degradation of these collagens by up-regulating MMP-1 and down-regulating TIMP-1 mRNA levels.