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目的 观察三七总皂甙(PNS)对大鼠肝脏缺血再灌注(IR)损伤中对线粒体的保护作用。方法 制作IR模型,阻断血运前将PNS经直接通道注入并保留于肝脏组织,并提取肝细胞线粒体,测定线粒体成分的超氧化物歧化酶(SOD)总活力、丙二醛(MDA)含量;观察电镜下的组织学改变;动态性对比观察再灌注早期上述指标的变化。结果 在再灌注90 min时实验组的SOD总活力明显低于相对应的对照组(P<0 .0 1) ,MDA含量明显低于相对应的对照组(P<0 .0 1) ,电镜观察表明实验组在再灌注90 m in时线粒体等超微结构的破坏明显比对照组轻。结论 PNS在大鼠肝脏IR早期主要通过直接清除自由基及提高线粒体SOD活力而减轻和预防大鼠IR肝细胞线粒体的损伤。
Objective To observe the protective effect of panax notoginseng saponins (PNS) on mitochondria during hepatic ischemia-reperfusion (IR) injury in rats. METHODS: An IR model was established to block the direct injection and retention of PNS into liver tissue before blood supply. Hepatocyte mitochondria were extracted and the total activity of superoxide dismutase (SOD) and malondialdehyde (MDA) content of mitochondrial components were measured. Observe the histological changes under electron microscope; observe the changes of the above indexes in the early stage of reperfusion. Results The total SOD activity of the experimental group was significantly lower than that of the corresponding control group (P<0.01) and the MDA content was significantly lower than that of the corresponding control group (P<0.01). Observations showed that the destruction of ultrastructure such as mitochondria in the experimental group was significantly lighter than that of the control group at a time of 90 min of reperfusion. Conclusion PNS can reduce and prevent mitochondrial damage in IR rat hepatocytes by directly scavenging free radicals and increasing mitochondrial SOD activity in rat liver IR.