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应用聚合酶链反应（ＰＣＲ）、单链构像多态性（ＳＳＣＰ）分析银染色法检测了６１例子宫颈癌、５例子宫颈间变、１４例慢性子宫颈炎组织中１６、１８型人乳头瘤病毒（ＨＰＶ１６、１８）感染及ｐ５３基因５、６、７、８外显子的变异。结果显示，子宫颈癌、子宫颈间变及慢性子宫颈炎组织中ＨＰＶ１６、１８阳性率分别为８２％、４０％及１４．３％（Ｐ＜０．０５），子宫颈癌组织中ｐ５３基因突变阳性率为２０％，而子宫颈间变及慢性子宫颈炎组织中未见有突变，其中１２例突变阳性的癌组织中９例呈ＨＰＶ阴性，３例呈ＨＰＶ阳性。提示ＨＰＶ１６、１８感染在子宫颈癌发生中起重要的病因学作用；ｐ５３基因变异可能是部分子宫颈癌变的一个重要原因；高危ＨＰＶ除了通过Ｅ６／ｐ５３蛋白复合物形成使ｐ５３蛋白失活的致癌途径外，可能还存在通过ｐ５３基因突变的致癌途径。
The use of polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) analysis of silver staining detected 61 cases of cervical cancer, 5 cases of cervical metaplasia, and 14 cases of chronic cervicitis tissue type 16 and 18 human papilloma Virus (HPV16, 18) infection and mutations in exon 5, 6, 7, and 8 of p53 gene. The results showed that the positive rate of HPV16, 18 in cervical cancer, cervical uterine cervix and chronic cervicitis tissues were 82%, 40% and 14.3%, respectively (P<0.05). The p53 gene mutation was found in cervical cancer tissues. The positive rate was 20%. There was no mutation in cervical uterine cervix and chronic cervicitis tissues. Nine of the 12 positive cancer tissues were HPV-negative and 3 were positive for HPV. It is suggested that HPV16, 18 infection plays an important etiological role in the development of cervical cancer; p53 gene mutation may be an important cause of partial cervical canceration; high-risk HPV may cause cancer inactivation of p53 protein in addition to the formation of E6/p53 protein complexes. Outside the pathway, there may also be a carcinogenic pathway through mutation of the p53 gene.