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目的：观察脂质体对日本脑炎病毒Ｅ糖蛋白的佐剂作用；方法：利用改进的振荡法，使亲和层析提取的日本脑炎病毒Ｅ糖蛋白包裹于脂质体中，制备出脂质体－Ｅ蛋白结合物，并分组免疫ＢＡＬＢ／ｃ小鼠；通过ＥＬＩＳＡ，血凝抑制试验（ＨＩ）和中和试验（ＮＴ）等指标观察．结果：脂质－Ｅ蛋白免疫组的特异性抗体水平明显高于游离Ｅ蛋白组；在两批病毒攻击保护性试验中，前者保护率分别为５０％和５７％，后者分别为３８％和３０％．结论：脂质体对日本脑炎病毒Ｅ糖蛋白有明显的佐剂作用．
OBJECTIVE: To observe the adjuvant effect of liposomes on Japanese encephalitis virus E glycoprotein.METHODS: The eukaryotic expression vector of Japanese encephalitis virus E glycoprotein extracted by affinity chromatography was encapsulated in liposomes by the improved oscillating method to prepare The liposome-E protein conjugates were immunized with BALB / c mice in groups; HI, HI and neutralization (NT) were observed by ELISA. Results: The specific antibody level of the lipid-E protein immunized group was significantly higher than that of the free E protein group. In the two batches of virus challenge protective test, the former protection rates were 50% and 57%, while the latter were 38% and 30%. Conclusion: Liposomes have obvious adjuvant effect on Japanese encephalitis virus E glycoprotein.