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AIM:To investigate the action of apoptosis in occurrence ofliver cacinomas in vivo and the biological effect of Solanumlyratum Thumb on BEL-7404 cell line inducing apoptosis invitro.METHODS:The apoptosis in the liver carcinoma wasdetected with terminal deoxynucl neotidyl transferasemediated dUTP nick end labelling(TUNEL);the cancer cellscultured in DMED medium ware treated with extract ofSolanum lyratum Thumb and observed under microscope,and their DNA was assayed by gel electrophoresis.RESULTS:In vivo apoptotic cells in the cancer adjacenttissues inceased;in vitro treatment of liver cancers withextract of Solanum lyratum Thumb could induce the cells tomanifest a typical apoptotic morphology.Their DNA wasfractured and a characteristic ladder pattem could be foundusing electrophoresis.CONCLUSION: In vivo the apoptosls of carcinomas was lower; maybe the cells divided quickly and then the cancers occurred. In the cancer adjacent tissues, the apoptosis pricked up, and in vitro Solarium tyratum Thumb could induce the apoptosis of BEL-7404 cells.
AIM: To investigate the action of apoptosis in occurrence of liver cacinomas in vivo and the biological effect of Solanumlyratum Thumb on BEL-7404 cell line inducing apoptosis invitro.METHODS: The apoptosis in the liver cancer was detected with terminal deoxynucl neotidyl transferasemediated dUTP nick end labelling ( TUNEL);the cancer cells cultured in DMED medium ware treated with extract ofSolanum lyratum Thumb and viewed under microscope,and their DNA was assayed by gel electrophoresis.RESULTS:In vivo apoptotic cells in the cancer adjacenttissues inceased;in vitro treatment of liver cancers withextract of Solanum lyratum Thumb can induce the cells tomanifest a typical apoptotic morphology.The DNA wasfractured and a characteristic ladder pattem could be foundusing electrophoresis.CONCLUSION: In vivo the apoptosls of carcinomas was lower; maybe the cells divided quickly and then the cancers occurred. In the Cancer adjacent tissues, the apoptosis pricked up, and in vitro Solarium tyr Atum Thumb could induce the apoptosis of BEL-7404 cells.