慢性门脉高压鼠胃微血管扩张与粘膜细胞动力学的关系

• 作 者: 武永忠 吴金生 赖大年 马庆久 何泽生 高德明
• 机 构: 第四军医大学唐都医院普通外科
• 出 处: 华人消化杂志
• 发表日期: 1998年09期
• 关键词: 门脉高压 血管扩张 粘膜

目的探讨慢性型门脉高压条件下胃微血管系统扩张瘀血与粘膜上皮细胞群体（ＧＭＥＣＧ）增殖动力学状态的关系．方法用３０ｇ／Ｌ硫代乙酰胺腹部ｓｃ法诱导大鼠慢性肝损害肝硬变及门脉高压，继用饥饿诱导法使大鼠发生门脉高压性胃粘膜出血（ＰＨＧＭＨ，ｎ＝３４），用形态测量法及微观放射自显影技术分期观察（注药４５ｄ，ＰＨＴＭＨⅠ，ｎ＝１４；注药６５ｄ，ＰＨＴＭＨⅡ，ｎ＝２０）模型鼠的胃粘膜微血管直径（ＤＧＭＭ）、粘膜下小静脉管径比（ＰＳｍＶＤ）、粘膜下厚度（ＳｍＴ）及粘膜上皮细胞群体标记指数（ＬＩ）的变化．结果①ＰＨＧＭＨ鼠出血率（９１２％）及严重性分级（２２７±０２０级）均大于对照（Ｐ＜００１），分期观察出血率（８５７％和９５０％）及严重性分级（１７９±００３级和２６０±０２５级）均大于对照（Ｐ＜００５和Ｐ＜００１）．②ＰＨＧＭＨ鼠ＤＧＭＭ（４０１μｍ±３０μｍ）、ＰＳｍＶＤ（０５０±００３）均大于对照（Ｐ＜００５和Ｐ＜００１）．分期观察ＰＨＧＭＨⅠ鼠ＤＧＭＭ和ＰＳｍＶＤ未达显著水平；而ＰＨＧＭＨⅡ鼠ＤＧＭＭ（４３８μｍ±４２０μｍ）、严重性分级（２６０±０２?

Objective To investigate the relationship between the proliferation of gastric mucosa epithelial cell population (GMECG) and the expansion of gastric capillary vasoconstriction in patients with chronic portal hypertension. Methods Chronic hepatic cirrhosis and portal hypertension were induced by 30g / L thioacetamide in abdomen in rats, followed by starvation-induced portal hypertension gastric mucosal hemorrhage (PHG-MH, n = 34 ), The morphological measurement and microscopic autoradiography staged observation (injection 45d, PHT MHl I, n = 14; injection of 65d, PHT MH II, n = 20) model mice gastric mucosal microvascular diameter (DGMM), submucosal venous diameter ratio (PSmVD), submucosal thickness (SmT) and mucosal epithelial cell population index (LI). Results ① The bleeding rate (912%) and severity (2.27 ± 0.20) in PHGMH group were higher than those in control group (P00101) 0%) and severity grading (179 ± 003 and 2  60 ± 025) were higher than the control (P <005 and P <001). (2) The DGMM (401μm ± 30μm) and PSmVD (050 ± 003) in PHGMH mice were all greater than those in control (P <005 and P <001). Stages of PHG  MH  I rat DGMM and PSmVD did not reach a significant level; while PHG  MH  Ⅱ DGMM mice (43  8μm ± 4  20μm), the severity of grading (2  60 ± 0  2?