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为考察卵转铁蛋白(OVT)对髓源性树突状细胞(BMDCs)成熟的影响。采用不同浓度OVT单独刺激和脂多糖(LPS)+不同浓度OVT共刺激第6 d小鼠BMDCs 48 h,通过倒置显微镜观察细胞形态,并用流式细胞仪测定各组CD11c+细胞比例以及DCs表面MHC-II,CD80和CD40分子表达量。结果表明,不同浓度OVT组刺激的DCs周围毛刺状突起增加,呈现典型的树突状细胞形态,且上调CD11c+细胞比例以及DCs表面MHC-II、CD80和CD40分子表达量。LPS+不同浓度OVT组中,低浓度(≤25μg/m L)组可提高CD11c+细胞比例,上调MHC-II、CD80和CD40表达量;高浓度(>25μg/m L)组则能下调CD11c+细胞比例和DCs表面MHC-II、CD80和CD40表达量。因此,不同浓度OVT均能刺激DCs的成熟;LPS+不同浓度OVT共刺激DCs,能显著提高DCs的成熟度,但呈现剂量依赖型,即低浓度时,两者表现出协同作用,高浓度时两者表现出拮抗作用。
To investigate the effect of ovotransferrin (OVT) on the maturation of myeloid dendritic cells (BMDCs). The BMDCs of 6th day were co-stimulated with different concentrations of OVT and lipopolysaccharide (LPS) + different concentrations of OVT for 48 h. The morphology of the cells was observed by inverted microscope. The proportion of CD11c + cells and the expression of MHC- II, CD80 and CD40 molecule expression levels. The results showed that the burr-like protrusions around DCs stimulated by OVT at different concentrations increased typical dendritic cell morphology, and increased the proportion of CD11c + cells and the expression of MHC-II, CD80 and CD40 on the surface of DCs. LPS + low concentration (≤25μg / m L) group could increase the proportion of CD11c + cells and up-regulate the expression of MHC-II, CD80 and CD40 in LPS + OVT group; And the expression of MHC-II, CD80 and CD40 on the surface of DCs. Therefore, different concentrations of OVT can stimulate the maturation of DCs; LPS + different concentrations of OVT co-stimulated DCs can significantly improve the maturity of DCs, but showed a dose-dependent, that is, low concentrations, the two showed synergistic effects, Who showed antagonism.