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目的:通过小鼠PM_(2.5)染毒模型,观察小鼠卵巢组织病理变化及雌激素受体(ER)、孕激素受体(PR)表达的变化,探讨PM_(2.5)对卵巢的毒性作用。方法:将小鼠随机分为对照组、低剂量组(0.2592μg/μl)、中剂量组(1.56695μg/μl)和高剂量组(3.4560μg/μl),采用改良型气管滴注法,分别给予PBS或PM_(2.5)混悬液30μl/次,每3天1次,间断滴注7次。观察其染毒期间的精神、体重变化。染毒结束后于动情间期称量小鼠体重及卵巢重量,计算卵巢脏器系数;观察小鼠卵巢病理变化。免疫组化法检测卵巢ER、PR的分布,Western blot法检测卵巢ER、PR蛋白的表达变化。结果:与对照组比较,高剂量染毒组小鼠第14~18天体重减轻,差异有统计学意义(P<0.05);中剂量组的卵巢湿重增加,差异有统计学意义(P<0.05);高剂量组的卵巢湿重和卵巢脏器系数增加明显,差异均有统计学意义(P<0.01,P<0.05)。随着PM_(2.5)染毒剂量的增加,卵巢皮质变薄,结构混乱,间质纤维化,颗粒细胞排列较对照组紊乱,卵泡数减少,卵泡闭锁相对增多,黄体细胞明显增大变空,炎细胞浸润增多等。免疫组化染色结果示,高剂量组卵巢组织中ER、PR表达显著低于PBS对照组(P<0.05)。Western blot法检测结果示,与对照组比较,随着染毒剂量的增加,卵巢中ER、PR蛋白表达逐渐降低,高剂量组中ER、PR蛋白表达均明显减少,差异有统计学意义(P<0.05)。结论:随着PM_(2.5)暴露剂量增加,小鼠卵巢组织损害明显,提示暴露于高浓度的PM_(2.5)环境可损害卵巢组织结构,进而可能破坏卵巢功能。
OBJECTIVE: To investigate the pathological changes of ovarian tissue and the expression of estrogen receptor (ER) and progesterone receptor (PR) in mouse PM_ (2.5) -induced model mice and to explore the toxic effects of PM_ (2.5) . Methods: The mice were randomly divided into control group, low dose group (0.2592μg / μl), middle dose group (1.56695μg / μl) and high dose group (3.4560μg / μl) Give PBS or PM_ (2.5) suspension 30μl / time, once every 3 days, intermittent infusion 7 times. Observed during the period of their mental, weight changes. The mice were weighed and the weight of ovary was weighed after the end of the exposure. The ovarian organ coefficient was calculated. The ovarian pathological changes were observed. The distribution of ER and PR in ovary was detected by immunohistochemistry and the expression of ER and PR in ovary was detected by Western blot. Results: Compared with the control group, the body weight of mice in the high-dose group decreased significantly on the 14th to the 18th day (P <0.05), while the wet weight of the middle-dose group increased, the difference was statistically significant (P < 0.05). The ovarian wet weight and ovarian organ coefficient of high-dose group increased significantly, the differences were statistically significant (P <0.01, P <0.05). With the increase of the dose of PM 2.5, ovarian cortex became thinner, the structure was disorganized, the interstitial fibrosis, the arrangement of granulosa cells were disordered compared with the control group, the number of follicles was reduced, follicular atresia was relatively increased, Inflammatory cell infiltration and so on. The results of immunohistochemistry showed that the expression of ER and PR in ovarian tissue of high dose group was significantly lower than that of PBS control group (P <0.05). The result of Western blot showed that the expression of ER and PR in ovary gradually decreased with the increase of exposure dose and the expression of ER and PR significantly decreased in high dose group compared with control group (P <0.05). CONCLUSION: With the increase of PM 2.5 exposure, the damage of ovary tissue in mice is obvious, which indicates that exposure to high concentration of PM 2.5 environment can damage the ovarian tissue structure, which may destroy the ovarian function.