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目的提高大黄虫丸质量标准。方法通过TLC对大黄虫丸中甘草进行鉴别;采用HPLC法同时测定大黄虫丸中大黄素和大黄酚的含量。结果TLC色谱中均能检出甘草;大黄素在0.0196~0.2352μg范围内呈良好线性关系(r=0.9999);大黄酚在0.0424~1.06μg范围内呈良好线性关系(r=0.9999);平均回收率大黄素为97.5%,RSD=1.48%,大黄酚为101.5%,RSD=1.57%。结论本法准确可靠,简便迅速,可用于大黄虫丸的质量控制。
Objective To improve the quality standard of Dahuangqichong Pills. Methods Radix Glycyrrhizae was identified by TLC. The content of rhubarbin and rhubarb was determined by HPLC method. Results Glycyrrhiza uralensis could be detected in TLC chromatograms. The rhizosteroids showed a good linearity in the range of 0.0196-0.2352 μg (r=0.9999). The rhubarb had a good linear relationship in the range of 0.0424-1.06 μg (r=0.9999). The average recovery rate for rhubarb was 97.5%, RSD was 1.48%, rhubarb was 101.5%, and RSD was 1.57%. Conclusion This method is accurate, reliable, simple and rapid, and can be used for quality control of Dahuangqichong Pills.