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目的研究Dukes A和B期大肠癌标本中VEGF基因表达及微血管密度与预后的关系。方法62例DukesA和B期大肠癌手术切除标本,采用RT-PCR法测定肿瘤组织中hVEGF165mRNA表达,以Westen蛋白印迹测定hVEGF蛋白表达,并测定标本微血管密度。所有患者接受5年以上随访。结果在随访期内出现局部复发或远处转移的患者与无复发或转移的患者相比,其VEGF基因表达及微血管数存在显著性差异。结论通过测定肿瘤标本中VEGF基因表达及微血管数可能预测Dukes A和B期大肠癌局部复发或远处转移的危险性。
Objective To investigate the relationship between VEGF gene expression, microvessel density and prognosis in Dukes A and B stage colorectal cancer specimens. Methods Sixty-two Dukes A and B stage colorectal cancer specimens were resected. The expression of hVEGF165 mRNA was detected by RT-PCR. Western blot was used to measure the expression of hVEGF protein and the microvessel density was measured. All patients received more than 5 years of follow-up. Results There were significant differences in VEGF gene expression and microvessel count between patients with local recurrence or distant metastasis and patients without recurrence or metastasis during the follow-up period. Conclusion It is possible to predict the risk of local recurrence or distant metastasis of Dukes A and B colorectal cancer by measuring VEGF gene expression and microvessel number in tumor specimens.