NF-κB “decoy”寡核苷酸对LPS诱导巨噬细胞产生NO及活性氧的影响

• 作 者: 王建 李金宝 任绪义 邓小明
• 机 构: 第二军医大学附属长海医院麻醉科,第二军医大学附属长海医院麻醉科,第二军医大学生物化学与分子生物学教研室,第二军医大学附属长海医院麻醉科 上海200433,,上海200433,上海200433,上海20
• 出 处: 中国病理生理杂志
• 发表日期: 2004年05期
• 关键词: 巨噬细胞 NF-kappa B 寡核苷酸类 一氧化氮 一氧化氮合酶 活性氧

目的 :探讨NF -κB“decoy”寡核苷酸 (ODNs)对脂多糖 (LPS)诱导小鼠巨噬细胞株J774 1产生一氧化氮 (NO)及活性氧的影响。方法 :通过体外细胞培养技术 ,观察转染NF -κB“decoy”ODNs对LPS刺激巨噬细胞产生NO和活性氧 (ROS)的影响 ,以及诱导型一氧化氮合酶 (iNOS)活力的变化。结果 :在LPS刺激的巨噬细胞中转染NF -κB“decoy”ODNs减少NO和ROS的产生 ,降低iNOS的活力。结论 :NF -κB“decoy”ODNs可以对抗LPS诱导巨噬细胞产生的ROS和NO ,可能与其特异性竞争抑制活化NF -κB结合位点有关。

Objective: To investigate the effects of NF-κB decoy oligodeoxynucleotides (ODNs) on nitric oxide (NO) and reactive oxygen species (ROS) production in murine macrophage J774 1 induced by lipopolysaccharide (LPS). Methods: The effects of NF-κB decoy ODNs on the production of NO and reactive oxygen species (ROS) by LPS-stimulated macrophages and the changes of inducible nitric oxide synthase (iNOS) activity were observed by cell culture in vitro. RESULTS: Transfection of NF-κB “decoy” ODNs in LPS-stimulated macrophages reduced NO and ROS production and decreased iNOS activity. CONCLUSION: NF-κB “decoy” ODNs can antagonize LPS-induced ROS and NO production by macrophages, which may be related to its specific competitive inhibition of activating NF-κB binding sites.