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目的研究浙江萧山医院婴幼儿童腹泻标本中人轮状病毒(Human Rotavirus)毒株的感染情况及G和P基因型流行特点。方法收集该院2009年8月至2010年8月腹泻儿童15233份粪便标本采用酶联免疫吸附试验、逆转录-巢式聚合酶链反应进行轮状病毒病原检测,将128份阳性标本进行VP7和VP4基本分型。结果 15233份婴幼儿腹泻标本中有2 706份标本为轮状病毒阳性,阳性率17.8%;男孩和女孩检出率差异无统计学意义,以6～12月龄段检出率最高;对128份阳性标本进行G血清分型和P基因分型,G1型53份(41.4%)、G3型38份(29.7%)、G1G3型17份(13.3%)、G未分型20份(15.6%);P[8]型72份(56.3%)、P[4]型16份(12.5%)、P[8]P[4]型3份(2.3%)、P未分型37份(28.9%),G血清型和P基因型的组合以G1P[8]为主,占29.7%(38/128)。结论浙江萧山医院A群轮状病毒G血清以G1型为主,其次为G3型,P基因型以P[8]型为主。
Objective To investigate the prevalence of human rotavirus strains and the prevalence of G and P genotypes in infantile diarrhea samples from Zhejiang Xiaoshan Hospital. Methods A total of 15233 specimens of diarrhea children from August 2009 to August 2010 in our hospital were collected for enzyme-linked immunosorbent assay (ELISA) and rotavirus pathogen detection by reverse transcription-nested polymerase chain reaction (PCR) VP4 basic classification. Results Among the 15,233 infantile diarrhea samples, 2 706 were positive for rotavirus, the positive rate was 17.8%. There was no significant difference in the detection rate between boys and girls and the highest detection rate was found in 6-12 months (41.4%), G3 (29.7%), G1G3 (17.3%), G (20.6%) were classified as G serotype ), 72 (56.3%) P [8], 16 (12.5%) P [4], 3 2.3% P [8] P [4] %). The combination of G serotype and P genotype was mainly G1P [8], accounting for 29.7% (38/128). Conclusion A group of rotavirus A seronegatives of Zhejiang Xiaoshan Hospital were G1 type, followed by G3 type, and P type was mainly P type [8].