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目的探讨内皮型一氧化氮合酶(NOS3)基因第4内含子27 bp 重复序列、Y 染色体HindⅢ酶切位点多态性与青年原发性高血压(EH)的关系。方法入选青年 EH 患者345例,正常人281名。提取白细胞 DNA,多聚酶链反应结合限制性内切酶(HindⅢ)方法检测多态性。结果对照组和 EH 组 HindⅢ(+)基因型分布分别为67.3%(107/159)和55.4%(102/184),两组间基因型差异有统计学意义(P=0.03),EH 患者 HindⅢ(-)基因型增多。对照组和 EH 组间 NOS3 4a/b 多态性各基因型和等位基因频率差异无统计学意义(P>0.05)。结论 Y 染色体 HindⅢ酶切位点多态性与青年 EH 有关。
Objective To investigate the relationship between the polymorphism of Y chromosome Hind Ⅲ restriction site and 27-bp repeat of intron 4 of NOS3 gene in young patients with essential hypertension (EH). Methods 345 cases of young EH patients, 281 normal subjects. Leukocyte DNA was extracted and the polymorphism was detected by polymerase chain reaction and restriction endonuclease (Hind Ⅲ). Results The distribution of Hind Ⅲ (+) genotype in control group and EH group was 67.3% (107/159) and 55.4% (102/184), respectively. There were significant differences in genotypes between the two groups (P = 0.03) (-) genotype increased. There was no significant difference in genotype and allele frequencies of NOS3 4a / b polymorphism between control group and EH group (P> 0.05). Conclusion Y chromosome Hind Ⅲ restriction site polymorphism and youth EH.