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通过EMS(Ethyl methylsulfonate,甲基磺酸乙酯)诱变豫谷1号获得一个遗传稳定的谷子小穗突变体si-sp1,该突变体突出表现为穗部变小,同时伴随有株高降低、单码小花数减少和根系变小等表现型变异。与野生型豫谷1号相比,突变体的穗长和株高分别降低了37.8%和9.0%,单穗粒重和单码小花数分别降低了40.3%和31.7%,但千粒重增加了20.2%。遗传分析表明,si-sp1突变性状由1对隐性基因控制。以si-sp1为母本、辽谷1号为父本构建的F2定位群体的隐性单株,将突变基因定位在8号染色体上CAAS8003与SSR1038间约11.02 M的距离内,为下一步精细定位并分离该基因奠定了基础。
A genetically stable millet spikelet, si-sp1, was mutagenized by EMS (Ethyl methylsulfonate) to obtain the stable spikelet spikelet of spikelet Millet. The mutant spikelet was smaller and accompanied by plant height reduction, Variation of phenotypic variation such as reduced number of single flower and smaller root system. Compared with the wild type Yugu 1, the panicle length and plant height of the mutants decreased by 37.8% and 9.0%, the grain weight per spike and the number of single-flower declines by 40.3% and 31.7%, respectively, but the 1000-grain weight increased by 20.2% . Genetic analysis showed that the si-sp1 mutation was controlled by one recessive gene. The recessive F2 locus population with Si-sp1 as the female parent and Liaogu 1 as the male parent locates the mutated gene at a distance of 11.02 M between CAAS8003 and SSR1038 on chromosome 8 for the next step of fine Positioning and separation of the gene laid the foundation.