β-FIBRINOGEN PROMOTER -455 G/A(HaeIII)POLYMORPHISM PREDICTION OF PLASMA FIBRINOGEN BUT NOT OF ISCHEM

• 作 者: 毕胜 王德生 李国忠 温世荣 潘尚哈
• 机 构: Institute of Cerebrovascular Diseases and Related Disorders,Department of Neurology, the First Clini
• 出 处: Chinese Medical Sciences Journal
• 发表日期: 2004年01期
• 关键词: minutes fibrinogen Wells Leach primer

Objective The -455 G/A(HaeIII)polymorphism of β-fibrinogen gene influences levels of plasma fibrinogen. We further investigated whether it influences the risk of ischemic cerebrovascular disease. Methods We accumulated 134 acute ischemic cerebrovascular disease(ICVD)cases and compared their -455 G/A status with a control group(n = 166). The β-fibrinogen gene -455 G/A polymorphism was analyzed for all subjects by PCR-RFLP with the restrictive enzyme HaeIII. Results Plasma fibrinogen was higher in AA homozygous participants(341 mg/dL)than in partici-pants carrying the G allele: GA(290 mg/dL), GG(298 mg/dL)in the control group. Plasma fibrinogen was also higher in AA homozygous patients(353 mg/dL)than in cases carrying the G allele: GA(287 mg/dL), GG(302 mg/dL)in the ICVD group. However, there was no significant association between β-fibrinogen gene -455 G/A polymorphism and ICVD group. Conclusions Although a small effect cannot be excluded, β-fibrinogen gene -455 G/A polymor-phism is an independent predictor of plasma fibrinogen, but not of ischemic cerebrovascular disease.

Objective The -455 G / A (HaeIII) polymorphism of β-fibrinogen gene affecting levels of plasma fibrinogen. We further有 whether it influences the risk of ischemic cerebrovascular disease. Methods We accumulated 134 acute ischemic cerebrovascular disease (ICVD) cases and compared their -455 G / A status with a control group (n = 166). The β-fibrinogen gene -455 G / A polymorphism was analyzed for all subjects by PCR-RFLP with the restrictive enzyme HaeIII. Results Plasma fibrinogen was higher in AA homozygous (341 mg / dL) than in partici-pants carrying the G allele: GA (290 mg / dL), GG (298 mg / dL) in the control group. Plasma fibrinogen was also higher in AA homozygous patients (353 mg / dL) than in cases carrying the G allele: GA (287 mg / dL), GG (302 mg / dL) in the ICVD group. However, there was no significant association between β-fibrinogen gene -455 G / A polymorphism and ICVD group. Conclusions Although a small effect can not be excluded, β-fibrinogen gene -455 G / A polymor-phism is an independent predictor of plasma fibrinogen, but not of ischemic cerebrovascular disease.