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目的:探讨荞麦黄酮(FB)对2型糖尿病(T2DM)大鼠糖脂代谢、胰岛素抵抗(IR)和炎症因子的影响及机制。方法:SD大鼠以高脂饮食喂养联合小剂量链脲佐菌素(STZ)腹腔注射制备T2DM大鼠模型。将大鼠随机分为正常对照组,模型组,阳性对照组(罗格列酮1 mg·kg-1,ig),FB低、中、高剂量组(FB100,200,400 mg·kg-1,ig)。测定空腹血糖(FBG)、血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C);放免法测定血清空腹胰岛素(FINS),计算胰岛素敏感指数(ISI);酶联免疫法测定肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、C-反应蛋白(CRP)。结果:模型组大鼠较正常对照组血清FBG,TC,TG,LDL-C,FINS,TNF-α,IL-6,CRP均显著升高(P<0.01),HDL-C显著下降(P<0.01);给予FBFL干预后糖脂代谢各指标明显改善,炎症因子TNF-α、IL-6、CRP水平明显降低(P<0.01,P<0.05),并呈一定剂量依赖性。结论:FB可能通过降低血清炎症因子TNF-α,IL-6,CRP水平,改善2型糖尿病大鼠糖脂代谢紊乱、胰岛素抵抗。
Objective: To investigate the effects and mechanisms of buckwheat flavonoids (FB) on glucose and lipid metabolism, insulin resistance (IR) and inflammatory cytokines in type 2 diabetic rats. Methods: SD rats were fed a high-fat diet combined with low-dose streptozotocin (STZ) intraperitoneal injection of T2DM rat model. The rats were randomly divided into normal control group, model group, positive control group (rosiglitazone 1 mg · kg -1, ig), FB low, middle and high dose group (FB 100, 200, 400 mg · kg -1, ig ). Fasting blood glucose (FBG), serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured; serum fasting insulin FINS) was calculated and insulin sensitivity index (ISI) was calculated. TNF-α, IL-6 and CRP were measured by enzyme-linked immunosorbent assay. Results: The levels of FBG, TC, TG, LDL-C, FINS, TNF-α, IL-6 and CRP in the model group were significantly higher than those in the normal control group (P < 0.01). The indexes of glycolipid metabolism were significantly improved after FBFL intervention. The levels of TNF-α, IL-6 and CRP in inflammatory cells were significantly decreased (P <0.01, P <0.05) and dose dependent. Conclusion: FB may ameliorate the disorder of glucose and lipid metabolism and insulin resistance in type 2 diabetic rats by decreasing the levels of serum inflammatory cytokines TNF-α, IL-6 and CRP.