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目的研究抗卵巢恶性肿瘤体外药敏测定与临床疗效的关系。方法收集卵巢恶性肿瘤２４例的３５份标本用改良的ＭＴＴ法研究卵巢恶性肿瘤对顺铂、卡铂、阿霉素、长春新碱、５－氟脲嘧啶、更生霉素的敏感性。结果不同个体之间对药物的敏感率差异有显著性（Ｐ＜０．０１）；单一药物的抑制率以铂类药物及阿霉素较高，顺铂的抑制率高于卡铂，联合用药较单一用药更为有效（Ｐ＜０．０１）。比较同一病例的实体瘤标本和腹水标本对药物的敏感率，发现二者密切相关（ｒ＝０．９２５）。测出敏感药物的１７例中１３例临床有效，未测出敏感药物的４例临床疗效较差，药敏组与不敏感组临床疗效差异有显著性（Ｐ＜０．０５）。体外药敏试验结果与临床疗效有良好的相关性（ｒ＝０．７８）。结论用改良ＭＴＴ法快速药敏选药可使化疗个体化，提高卵巢恶性肿瘤的治疗效果。
Objective To study the relationship between antimicrobial susceptibility and clinical efficacy of anti-ovarian cancer in vitro. Methods Twenty-five specimens from 24 patients with ovarian malignant tumors were collected and their susceptibility to cisplatin, carboplatin, doxorubicin, vincristine, 5-fluorouracil and dactinomycin were studied by modified MTT assay. Results There was a significant difference (P <0.01) in the sensitivity of different individuals to the drug. The single drug inhibition rate was higher in platinum drugs and doxorubicin, cisplatin than in carboplatin, More than a single drug is more effective (P <0.01). Comparison of the same case of solid tumor specimens and ascites specimens of drug sensitivity, found that both are closely related (r = 0.925). Among the 17 cases with sensitive drugs, 13 cases were clinically effective and 4 cases without any sensitive drugs had poor clinical curative effect. There was significant difference (P <0.05) in clinical curative effect between drug-sensitive group and non-sensitive group. In vitro susceptibility test results and clinical efficacy have a good correlation (r = 0.78). Conclusions The rapid MTT assay using modified MTT method can make chemotherapy individual and improve the therapeutic effect of ovarian cancer.