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盐胁迫是盐碱地高粱生产中重要的限制因子。为了更好地了解盐胁迫下高粱苗期耐盐分子遗传机制,服务于高粱耐盐分子育种,本研究利用粒用高粱石红137和甜高粱L-甜杂交衍生的181个重组自交系群体,通过对正常生长条件(0.0%NaCl)和盐胁迫(0.3%NaCl)下的苗高、苗鲜重和苗干重进行调查,利用复合区间作图方法,在正常生长条件下分别检测到3和2个控制苗高和苗干重的QTL;在盐胁迫下分别检测到7、2和2个控制苗高、苗鲜重和苗干重的QTL。其中控制苗高的qSH1-1,在正常生长条件和盐胁迫下均可检测到。与正常生长条件相比,检测到6个盐胁迫下控制苗高的特异表达位点。苗鲜重和苗干重在正常生长条件和盐胁迫下受不同QTL控制。本研究表明,长时间低度盐胁迫可显著抑制高粱幼苗生长,在盐胁迫下控制高粱苗高、苗鲜重和苗干重QTL的表达具有较强的环境特异性,而控制高粱苗高的qSH1-1和qSH7-2在高粱耐盐遗传改良中将发挥重要作用。
Salt stress is an important limiting factor in the production of sorghum. In order to better understand the molecular mechanisms of salt tolerance in sorghum seedlings under salt stress and to serve the salt-tolerant molecular breeding of sorghum, 181 recombinant inbred lines derived from sorghum-red 137 and sweet sorghum L-sweet hybrids The seedling height, seedlings fresh weight and seedling weight under normal growth condition (0.0% NaCl) and salt stress (0.3% NaCl) were investigated. The composite interval mapping method was used to detect 3 And two QTLs for controlling seedling height and seedling dry weight. Seven, two and two QTLs for controlling seedling weight, seedling fresh weight and seedling weight were detected under salt stress. Among them, qSH1-1, which controls seedling height, can be detected under normal growth conditions and salt stress. Compared with the normal growth conditions, six salt stress-specific control sites were detected. Fresh weight and dry weight of seedlings were controlled by different QTLs under normal growth conditions and salt stress. The results showed that low salt stress for a long time could significantly inhibit the growth of sorghum seedlings and control the sorghum seedling height, seedling fresh weight and dry weight QTL expression under the salt stress, qSH1-1 and qSH7-2 play an important role in genetic improvement of sorghum salt tolerance.