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目的优化高良姜的乙醇提取工艺。方法建立高效液相色谱法,同时测定高良姜中5-羟基-7-(4-羟基-3-甲氧基苯基)-1-苯基-3-庚酮、高良姜素、山柰素、7-(4-羟基-3-甲氧基苯基)-1-苯基-4-烯-3-庚酮和1,7-二苯基-5-羟基-3-庚酮5种成分,采用L9(34)正交试验法,以上述5种成分含有量的综合评分为指标,考察乙醇体积分数、提取时间、提取次数和料液比4个影响因素,优选高良姜的醇提工艺。结果提取次数对高良姜的提取工艺有显著性影响,优选出的提取工艺为加10倍量80%乙醇,提取3次,每次1 h;高良姜中5种成分的转移率分别为5-羟基-7-(4-羟基-3-甲氧基苯基)-1-苯基-3-庚酮91.7%、高良姜素92.3%、山柰素85.0%、7-(4-羟基-3-甲氧基苯基)-1-苯基-4-烯-3-庚酮94.8%、1,7-二苯基-5-羟基-3-庚酮93.9%。结论多成分综合评分法优选出的提取工艺能对高良姜药材进行较完全地提取,可用于在线监控。
Objective To optimize the ethanol extraction process of galangal. Methods High performance liquid chromatography (HPLC) was used to determine the contents of 5-hydroxy-7- (4-hydroxy-3-methoxyphenyl) -1-phenyl-3-heptanone, galangin, , 5- (4-hydroxy-3-methoxyphenyl) -1-phenyl-4-ene-3-heptanone and 1,7-diphenyl-5-hydroxy-3-heptanone , Using L9 (34) orthogonal test to take the above five ingredients content of the comprehensive score as an index to examine the ethanol volume fraction, extraction time, the number of extraction and the ratio of liquid to liquid factors, preferably Alkalis alcohol extraction process . The results showed that the extraction times had significant effects on the extraction of Alpinia officinarum. The optimal extraction process was extraction with 10 times of 80% ethanol and extraction for 3 times for 1 hour. The transfer rates of 5 components in Alpinia officinarum were 5- 91.7% of hydroxy-7- (4-hydroxy-3- methoxyphenyl) -1-phenyl- 3-heptanone, 92.3% of galangin, 85.0% -methoxyphenyl) -1-phenyl-4-ene-3-heptanone, 93.9% of 1,7-diphenyl-5-hydroxy-3-heptanone. Conclusion The extraction process optimized by the multi-component comprehensive score method can extract the alpinia officinalis more completely and can be used for on-line monitoring.