论文部分内容阅读
利用9对引物对46个品种或品系的DNA进行扩增,共得到39条多态性谱带,以相似系数和遗传距离矩阵,采用类平均法进行聚类分析,46份材料在相似系数0.33时,被分为两大类,I类为海岛棉,II类为陆地棉。II类组中陆地棉在相似系数0.568时,被分为2个亚组。亚组1为海陆杂交低代材料,具有陆地棉和海岛棉综合特征较多,从DNA扩增的谱带看,多是海陆杂合带,并有较多海岛棉带型。亚组2除品系冀031823和冀04425为纯陆地棉外,具有海岛棉优质基因的陆地棉渐渗系被分成不同的小组。扩增结果表明渐渗系主要遗传背景为陆地棉,个别性状来源于海岛棉。由于渐渗位点和基因不同,从而造成一定的差异,被聚类到不同小组。该研究为这些品系利用和新品种、杂交种培育的亲本选择提供了初步的理论依据。
Nineteen pairs of primers were used to amplify the DNA of 46 cultivars or lines. A total of 39 polymorphic bands were obtained. Clustering analysis was performed using the method of cluster average with similarity coefficient and genetic distance matrix. Among 46 materials, the similarity coefficient was 0.33 Are classified into two broad categories, type I as sea island cotton and type II as upland cotton. Upland cottons in Group II were divided into 2 subgroups at a similarity coefficient of 0.568. Subgroup 1 is a lowland and crossbred material, which has more comprehensive characteristics of upland cotton and sea-island cotton. Most of them are land-sea hybrid belt and more island cotton belt type. Subgroup 2, except that lines 031823 and Hebei 04425 were pure upland cotton, the intrusive introgression lines of upland cotton with high-quality island cotton genes were divided into different groups. The amplification results showed that the major genetic background of introgression lines was Upland cotton, and some traits originated from island cotton. As the infiltration site and genes are different, resulting in some differences, are clustered into different groups. The research provided a preliminary theoretical basis for the utilization of these lines and the selection of parents for cultivating new varieties and hybrids.