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应用淋巴细胞单克隆抗体(McAb)的Panning法或亲和层析分纯 CD_4~+、CD_8~+、B细胞和非CD_4~+细胞,也应用PHA、ConA、PWM和LPS分别激活淋巴细胞,然后用~3H-TdR按入、集落形成及羟基磷灰石方法,探讨了各亚群淋巴细胞的调节功能及其辐射效应.实验结果表明PWM能激活CD_4~+、非CD_4~+和B细胞,PWM细胞能诱导LPS细胞转化,其中一种细胞受照即失去协同作用,PWM细胞受照影响更严重.ConA能激活CD_4~+和CD_8~+细胞,两者均可抑制LPS细胞转化,并能互相增强抑制效应,受照后消失.辐射敏感性比较:ConA细胞和PHA细胞大于PWM细胞,PWM细胞大于LPS细胞,CD_8~+细胞和B细胞大于CD_4~+细胞。
CD_4 ~ +, CD_8 ~ +, B cells and non-CD_4 ~ + cells were also isolated by Panning assay or affinity chromatography using lymphocyte monoclonal antibody (McAb). Lymphocytes were also activated by PHA, The regulatory function and radiation effects of lymphocytes in different subpopulations were investigated by ~ 3H-TdR incorporation, colony formation and hydroxyapatite method. The experimental results show that PWM can activate CD_4 ~ +, non-CD_4 ~ + and B cells , PWM cells can induce the transformation of LPS cells, one of the cells lost the synergistic effect of irradiation, and the PWM cells were more affected by irradiation.ConA can activate CD_4 ~ + and CD_8 ~ + cells, both of which can inhibit the transformation of LPS cells and Can enhance the inhibitory effect to each other and disappear after irradiation.Compared with the radiation sensitivity, ConA cells and PHA cells are larger than the PWM cells, the PWM cells are larger than the LPS cells, the CD_8 ~ + cells and the B cells are larger than the CD_4 ~ + cells.