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目的 观察纤维蛋白胶 (FG)包裹嗅神经鞘细胞 (OECs)植于成年大鼠脊髓全横断断端间隙内对损伤轴突再生的促进作用。 方法 成年雄性SD大鼠 16只 ,行脊髓T11节段全横断术。FG OECs组于间隙内移植FG包裹的OECs ,FG对照组移植等量的FG ,OECs对照组移植等量DF12 OECs悬液 ,单纯全切对照组未做任何移植 ,每组动物 4只。术后 2周处死动物 ,脊髓损伤区冰冻水平切片 ,荧光显微镜下观察OECs的存活及迁移 ,并行抗神经丝 (NF)和生长相关蛋白 4 3(GAP 4 3)抗体免疫组织化学染色。 结果 OECs及单纯全切对照组脊髓两断端连接较差 ,而FG对照组及FG OECs组两断端连接良好。FG、OECs及单纯全切对照组损伤区可见少量NF及GAP 4 3免疫反应纤维 ;FG OECs组间隙内OECs大量存活 ,并迁移至两侧脊髓实质内 ,大量NF及GAP 4 3免疫反应纤维通过间隙。 结论 FG包裹的OECs可在成年大鼠脊髓全横断断端间隙内存活并迁移至脊髓实质内 ,促进损伤轴突的再生
Objective To observe the promoting effect of fibrin glue (FG) - entrapped olfactory ensheathing cells (OECs) implanted into the gap between the transected severed spinal cord in adult rats. Methods Sixteen adult male Sprague-Dawley rats underwent transection of T11 segment of the spinal cord. The FG OECs group were transplanted with FG-encapsulated OECs in the interstitium. The FG control group was transplanted with the same amount of FG. The OECs control group was transplanted with the equal amount of DF12 OECs suspension. Only four rats in each group were not transplanted. The animals were sacrificed 2 weeks after operation and the spinal cord injury area was frozen. The survival and migration of OECs were observed under a fluorescence microscope. The anti-neurofilament (NF) and growth-associated protein 43 (GAP 4 3) antibodies were immunohistochemically stained. Results There was a poor connection between the two ends of the spinal cord in OECs and purely-cut control groups, but there was a good connection between the FG control group and the FG OECs group. A small amount of NF and GAP 4 3 immunoreactive fibers were found in the damaged area of FG, OECs and simple control group. OECs in FGOECs group survived in a large amount and migrated to both sides of the spinal cord parenchyma. A large number of NF and GAP 4 3-reactive fibers passed gap. Conclusion The FG-encapsulated OECs can survive and migrate into the parenchyma of the spinal cord in the spinal cord transectional spaces of adult rats, and promote the regeneration of injured axons