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AIM To identify the property of dendritic cells(DCs)ofperipheral blood monocytes(PBMC)in patients withchronic HBV infection.METHODS Twenty patients with persistent HBV infectionwere included in this study,10 healthy subjects beingused as a control group.The peripheral bloodmononuclear cells(PBMC)of T cell-depleted populationswere incubated and induced into mature dendritic cells inthe RPMI-1640 medium in the presence of cytokines GM-CSF,IL-4,FLt-3,TNF-α and 100 mL·L~(-1) of fetal calf serumfor a total of 10-12 days.The expressions of surfacemarkers on DCs were evaluated using flow cytometricanalysis.ELISA method was used to determine thecytokine levels of interleukin-12(IL-12)and IL-10 in thesupernatant produced by DCs.For detection of theetimulatory capacity of DCs to T cell proliferation,mytomycin C-treated DC were incubated with allogenic Tcells.RESULTS A typical morphology of mature DCs fromhealthy subjects and HBV-infected patients was induced inin vitro incubation,but the proliferation ability andcellular number of DCs from HBV-infected patientssignificantly decreased compared with healthyindividuals.In particular,the expression levels of HLA-DR,CD80(B7-1)and CD86(B7-2)on DC surface frompatients were also lower than that from healthy individuals(0.46 vs 0.92 for HLA-DR,0.44 vs 0.88 for CD80 and 0.44vs 0.84 for CD86,P<0.05).The stimulatory capacity andproduction of IL-12 of DCs from patients in allogenic mixedlymphocyte reaction(AMLR)significantly decreased,butthe production level of nitric oxide(NO)by DCssimultaneously increased compared with healthy subjects(86 ± 15 vs 170 ±22 μmol·L~1,P<0.05).CONCLUSION The patients with chronic HBV infectionhave the defective function and immature phenotype ofdendritic cells,which may be associated with the inabilityof efficient presentation of HBV antigens to host immunesystem for the clearance of HBV.
AIM To identify the property of dendritic cells (DCs) ofperipheral blood monocytes (PBMC) in patients with chronic HBV infection. METHODS Twenty patients with persistent HBV infectionwere included in this study, 10 healthy subjects beingused as a control group. The peripheral blood mononuclear cells (PBMC ) of T cell-depleted populationswere incubated and induced into mature dendritic cells inthe RPMI-1640 medium in the presence of cytokines GM-CSF, IL-4, FLt-3, TNF-α and 100 mL·L -1 of fetal calf serum for a total of 10-12 days. The expressions of surfacemarkers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the etimulatory capacity of DCs to T cell proliferation, mytomycin C-treated DC were incubated with allogenic T cells. RESULTS A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proli fection ability andcellular number of DCs from HBV-infected patientssignificantly decreased compared with healthyindividuals. In particular, the expression levels of HLA-DR, CD80 (B7-1) and CD86 (B7-2) on DC surface frompatients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs. 0.84 for CD86, P <0.05). The stimulatory capacity andproduction of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCssimultaneously increased compared with healthy subjects (86 ± 15 vs 170 ± 22 μmol·L ~ 1, P <0.05) .CONCLUSION The patients with chronic HBV infectionhave the defective function and immature phenotype of dendritic cells , which may be associated with the inability of efficient presentation of HBV antigens to host immunesystem for the clearance of HBV.