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目的:测定11种抗菌药物对鼠疫耶尔森菌（简称鼠疫菌）的最低抑菌浓度（minimum inhibitory concentration，MIC），建立鼠疫菌MIC测定方法，掌握常用抗生素对鼠疫菌的抑菌范围，为鼠疫的临床治疗提供基线数据。方法:根据美国临床和实验室标准协会（Clinical Labor Standard Institution，CLSI）药敏试验方法中的琼脂稀释法，分别测定氧氟沙星、环丙沙星、复方新诺明（甲氧苄啶-磺胺甲恶唑）、硫酸卡那霉素、硫酸链霉素、头孢曲松钠、氨苄青霉素钠、氯霉素、盐酸壮观霉素、头孢呋辛钠、盐酸四环素共11种抗菌药物对118株鼠疫菌的MIC，并计算MICn 50、MICn 90（能抑制50%、90%细菌生长的最低药物浓度）。以纸片扩散法的检测结果作为对照观察两者的一致性。118株鼠疫菌分离自青海省鼠疫自然疫源地，由青海省地方病预防控制所保存。n 结果:检测的118株鼠疫菌，未发现具有单个或多个抗菌药物抗性的菌株，与纸片扩散法结果一致，并获得11种抗菌药物对118株菌的MICn 50、MICn 90。n 结论:成功建立了鼠疫菌MIC测定方法，该方法能高通量测定抗菌药物对鼠疫菌的MIC，评价鼠疫菌对抗生素的敏感性，是一种高效、经济、实用的实验手段。“,”Objective:To establish the minimum inhibitory concentration (MIC) detection method of n Yersinia pestis by determining MIC of 11 kinds of antibiotics against n Yersinia pestis, to master the inhibition range of common antibiotics on n Yersinia pestis, and provide baseline data for clinical treatment of plague.n Methods:According to Clinical Labor Standard Institution (CLSI), the agar plate dilution method was used to determine the MIC of 11 kinds of antibiotics against 118 strains of n Yersinia pestis, including ofloxacin, ciprofloxacin, trimethoprim-sulfamethoxazole, kanamycin, streptomycin, ceftriaxone, ampicillin, chloramphenicol, spectinomycin, cefuroxime and tetracycline. MICn 50 and MICn 90 (the minimum concentration of drug which could inhibit 50% and 90% of bacterial growth) were calculated. The consistency was observed by comparing the results with those of the disk diffusion method. One hundred and eighteen strains of n Yersinia pestis were isolated from natural plague foci of Qinghai Province and preserved by Qinghai Institute for Endemic Disease Prevention and Control.n Results:Among 118 strains of n Yersinia pestis tested, no single or multiple strains of n Yersinia pestis resistant to 11 kinds of antibiotics were found, which was consistent with the results of the disk diffusion method. The MICn 50 and MICn 90 of 11 kinds of antibiotics against 118 strains of n Yersinia pestis were obtained.n Conclusions:The MIC detection method of n Yersinia pestis is successfully established. This method can be used to measure the MIC of antibiotics against n Yersinia pestis in high throughput and evaluate the sensitivity of n Yersinia pestis to antibiotics. It is an efficient, economical and practical experimental method.n