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目的：观察地塞米松对低温冷冻后视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞内Ｃａ２＋浓度的影响。方法：在－７０℃条件下，冷冻ＲＰＥ细胞３０秒，分别加入４０、６０、１００、１５０、２００μｇ／ｍｌ的地塞米松后，应用Ｆｕｒａ－２／ＡＭ荧光负荷技术，测定ＲＰＥ细胞Ｃａ２＋浓度。结果：地塞米松在４０μｇ／ｍｌ～６０μｇ／ｍｌ浓度范围可使冷冻后ＲＰＥ细胞内Ｃａ２＋增高１８．６％～２９．８％，在１５０μｇ／ｍｌ～２００μｇ／ｍｌ可降低细胞内Ｃａ２＋浓度２８．４％～３５．２％。结论：地塞米松对冷冻后ＲＰＥ细胞内Ｃａ２＋的影响表现为双重作用，即低浓度的促进作用和高浓度的抑制作用，提示临床用药应在冷冻瞬间冲击治疗。
Objective: To observe the effect of dexamethasone on intracellular Ca2 + concentration in retinal pigment epithelial cells (RPE) after cryopreservation. Methods: RPE cells were frozen at -70 ℃ for 30 seconds. After adding 40, 60, 100, 150, 200μg / ml dexamethasone respectively, Fura-2 / AM fluorescence load technique was used to determine the concentration of Ca2 + in RPE cells. Results: Dexamethasone increased the intracellular Ca2 + of frozen RPE cells by 18.6% ~ 29.8% at the concentration range of 40μg / ml ~ 60μg / ml, while the intracellular Ca2 + concentration decreased by 150μg / ml ~ 200μg / ml. 4% ~ 35.2%. Conclusion: The effect of dexamethasone on intracellular Ca2 + in RPE cells after cryopreservation is twofold, that is, the effect of low concentration and the high concentration of dexamethasone can inhibit the RPE cells.