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目的:采用主成分分析神农香菊挥发油成分,为神农香菊药理作用的物质基础提供科学依据。方法:采集15批次神农香菊样品,水蒸气蒸馏法提取挥发油,并通过气相色谱-质谱联用(GC-MS)对其分析,实验数据通过主成分分析(Principal Component Analysis,PCA)进行综合评价。结果:最终通过5个主成分对原有指纹图谱中共有的23个指标信息作出概括,包含原指标88.966%信息量。第一主成分PC_1可作为神农香菊挥发油平喘作用的描述指标,第二主成分PC_2可作为神农香菊挥发油镇痛作用的描述指标,第三主成分(PC_3)可作为神农香菊挥发油止咳作用的描述指标。结论:本研究通过主成分分析方法对神龙香菊挥发油的共有成分进行分析,为神农香菊挥发油平喘作用、镇痛作用、止咳作用的物质基础提供了理论参考和实践探索。
OBJECTIVE: To analyze the volatile oil components of Shennongxiangju by principal component analysis (PCA) and provide the scientific basis for the material basis of pharmacological effects of Shennongxiangju. Methods: Fifteen batches of Shennongxiangju were collected and the volatile oil was extracted by steam distillation. The results were analyzed by GC-MS and the experimental data were integrated by Principal Component Analysis (PCA) Evaluation. Results: Finally, the five principal components were used to summarize the 23 indicators shared by the original fingerprinting, covering 88.966% of the original index. The first principal component PC_1 can be used as a descriptive index of the antiasthmatic effect of Shennongxiangju volatile oil, the second principal component PC_2 can be used as a description index of the analgesic effect of Shennongxiangju volatile oil, and the third principal component (PC_3) Description of the role of indicators. Conclusion: In this study, the principal component analysis of the common constituents of the volatile oil of the chrysanthemum saxifrage, Shennong fragrant essential oil volatile oil anti-asthmatic effect, analgesic effect, cough effect of the material basis provided a theoretical reference and practical exploration.