论文部分内容阅读
目的 :构建乙型肝炎病毒 (HBV)基因疫苗 pCR3.1 S ,观察重组人白细胞介素 2 (rhIL 2 )作为佐剂对其诱导BALB/c小鼠产生免疫应答的影响。方法 :以ELISA法检测免疫小鼠血清抗HBs抗体 ,另用3 H TdR掺入法测定淋巴细胞增殖活性 ,初步研究不同组的体液和细胞免疫应答。结果 :rhIL 2组免疫小鼠抗HBs抗体和淋巴细胞增殖活性与对照组比较差异有统计学意义 (P <0 .0 5 )。结论 :HBV基因疫苗可诱发BALB/c小鼠产生良好的免疫应答 ,rhIL 2作为佐剂可增强其免疫效应。
Objective: To construct the hepatitis B virus (HBV) gene vaccine pCR3.1S and observe the effect of recombinant human interleukin 2 (rhIL 2) as an adjuvant on the immune response induced by BALB / c mice. Methods: Serum anti-HBs antibodies were detected by ELISA. Lymphocyte proliferative activity was measured by 3 H TdR incorporation, and the humoral and cellular immune responses of different groups were preliminary studied. Results: The anti-HBs antibody and lymphocyte proliferation activity of rhIL 2 immunized mice was significantly different from that of the control group (P <0.05). Conclusion: HBV gene vaccine can induce good immune response in BALB / c mice. As an adjuvant, rhIL 2 can enhance its immune response.