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目的探讨牛磺酸对缺氧引起的神经胶质细胞凋亡的抑制作用方法原代培养神经胶质细胞并制作细胞缺氧模型,实验分为空白对照组、缺氧模型组、缺氧+牛磺酸组。细胞缺氧6 h后加入牛磺酸3 mmol/L继续培养24 h和48 h。采用Annexin V-FITC/PI双染色流式细胞术检测细胞凋亡率,RT-PCR检测凋亡相关基因表达水平。结果缺氧组48 h早期凋亡率为(2.48±0.92)%,中晚期凋亡率为(2.97±0.75)%,均显著高于对照组的(1.02±0.44)%(P<0.05);牛磺酸48 h组早期凋亡率为(1.39±1.03)%,中晚期凋亡率为(1.62±0.1)%,均显著低于缺氧组(P<0.05)。缺氧组Bax mRNA相对表达量均显著高于对照组和牛磺酸组(P<0.01);牛磺酸48 h组Bcl-2 mRNA相对表达量比缺氧组升高2倍(P<0.01);牛磺酸组HIF-1αmRNA表达量均比缺氧组升高显著(P<0.01)。结论牛磺酸有助于抑制缺氧所致的神经胶质细胞早期和中晚期凋亡率。
Objective To investigate the inhibitory effect of taurine on hypoxia-induced glial cell apoptosis.Methods Primary cultured glial cells were cultured in vitro and hypoxia model was established. The experiment was divided into blank control group, hypoxia model group, hypoxia + Sulfonic acid group. Cells were exposed to 3 mmol / L taurine for 24 h and 48 h after hypoxia for 6 h. The apoptotic rate was detected by Annexin V-FITC / PI double staining flow cytometry, and the apoptosis related gene expression was detected by RT-PCR. Results The apoptotic rate in early hypoxia group was (2.48 ± 0.92)% at 48 h, and (2.97 ± 0.75)% in late hypoxia group was significantly higher than that of control group (1.02 ± 0.44)% (P <0.05). The early apoptosis rate of taurine in 48 h group was (1.39 ± 1.03)%, and the late apoptosis rate was (1.62 ± 0.1)%, which were significantly lower than those in hypoxia group (P <0.05). The relative expression of Bax mRNA in hypoxia group was significantly higher than that in control group and taurine group (P <0.01). The relative expression of Bcl-2 mRNA in hypoxia group was increased by 2 times (P <0.01) ; The expression of HIF-1αmRNA in taurine group was significantly higher than that in hypoxia group (P <0.01). Conclusion Taurine helps to inhibit the early and late apoptotic rate of glial cells induced by hypoxia.