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目的探讨砷化物对人胃癌细胞的抑制及放射增敏作用。方法分别用不同浓度的三氧化二砷(As_2O_3)及不同剂量的60Coγ射线对体外传代培养的人胃癌细胞株SGC-7901进行处理。将每个细胞株分成六组,即对照组、观察A组、观察B组、照射组、联合A组、联合B组,采用荧光倒置显微镜及MTT法对细胞增殖情况进行观察和细胞计数;使用流式细胞仪对细胞凋亡情况进行检测。结果六组细胞计数、增殖抑制率、细胞凋亡率对比较,差异有统计学意义(P<0.05)。结论 As_2O_3对人胃癌细胞有一定的抑制作用,随着浓度的增加及时间的增长,其抑制作用增强,同时具有一定的放射增敏作用。
Objective To investigate the inhibitory effect of arsenide on human gastric cancer cells and radiosensitization. Methods SGC-7901 human gastric cancer cell line SGC-7901 cultured in vitro with different concentrations of arsenic trioxide (As 2 O 3) and different doses of 60Co γ-rays were treated. Each cell line was divided into six groups: control group, observation group A, observation group B, irradiation group, combination A group, combined with group B, the cell proliferation was observed by fluorescent inverted microscope and MTT method; Flow cytometry to detect apoptosis. Results Six groups of cell count, proliferation inhibition rate, apoptosis rate comparison, the difference was statistically significant (P <0.05). Conclusion As2O3 has a certain inhibitory effect on human gastric cancer cells. With the increase of concentration and time, As2O3 enhances its inhibitory effect and at the same time it has certain radiosensitization effect.