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选用6周龄雄性BALB/C小鼠(体重22～25g)和3月龄雄性Wistar大鼠(体重300～400g)。每只小鼠一次性腹腔注射2-乙基己酸(放射性标记后为2-~(14)C-EHA)5μCi,分别于注射后0.5、1、6小时以二氧化碳麻醉、液氮冷冻动物,再以羧甲基纤维素包埋,制成20/μm厚切片;切片置—20℃室内冻干一天,放于胶片上,置—20℃环境中暴露6周,整个显影过程按常规暗室技术处理。上述放射性自显影方法为Ullberg(1977)所介绍。每只大鼠腹腔注射2-~(14)C-EHA 2μCi,分别于注射后2、6、24小时以二氧化碳麻醉动物,断头处死,
Six-week-old male BALB / C mice (weighing 22-25 g) and 3-month-old male Wistar rats (weighing 300-400 g) were selected. Each mouse was intraperitoneally injected with 5μCi of 2-ethylhexanoic acid (2 ~ (14) C-EHA after radiolabeling), anesthetized with carbon dioxide 0.5,1,6 hours after injection, And then carboxymethyl cellulose embedding, made of 20 / μm thick sections; slice set at -20 ℃ indoor lyophilized one day, placed on film, set at -20 ℃ environment for 6 weeks, the entire development process according to conventional darkroom technology deal with. The above method of autoradiography is described by Ullberg (1977). Each rat was intraperitoneally injected with 2 ~ (14) C-EHA 2μCi, animals were anesthetized with carbon dioxide at 2,6,24 hours after injection,