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采用常规的分子生物学技术 ,从小鼠骨髓细胞中克隆了含信号肽序列的SCF ,并构建了表达载体。序列分析表明所克隆的SCF序列与文献报道的一致 ,构建的表达载体经鉴定正确。
SCF containing the signal peptide sequence was cloned from mouse bone marrow cells using conventional molecular biology techniques and an expression vector was constructed. Sequence analysis showed that the cloned SCF sequence was consistent with that reported in the literature, and the constructed expression vector was identified correctly.