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为了让学生在学习“食用真菌”知识时,能观察到菌丝生长和子实体发育的过程,经过反复实验,采用金针菇培养皿平面培养法,可在较短时间内达到观察目的,效果良好。培养基配制去皮马铃薯煮出液20毫升,葡萄糖2克,硫酸镁0.05克,磷酸二氢钾0.1克,琼脂2克,水100毫升。把配制好的培养基装入三角烧瓶中,加棉塞外包牛皮纸,经1.5公斤/厘米~2高压灭菌25分钟,冷却备用。并将洗净的中号(直径9厘米左右)培养皿若干套也同时高压灭菌。
In order to enable students to learn the “edible fungus” knowledge, can be observed mycelial growth and fruiting body development process, after repeated experiments, Flammulina velutipes flat culture method can be achieved in a short period of observation purposes, the effect is good. Medium Preparation peeled potato cooking liquid 20 ml, glucose 2 g, 0.05 g of magnesium sulfate, potassium dihydrogen phosphate 0.1 g, agar 2 g, water 100 ml. The prepared medium into the conical flask, add tampon outsourcing kraft paper, 1.5 kg / cm ~ 2 autoclave for 25 minutes, cooling standby. And washed medium (diameter of about 9 cm) Petri dishes set at the same time several autoclave.