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目的探讨红芪复方预处理对大鼠心肌缺血再灌注损伤的保护作用。方法健康雄性SD大鼠50只,随机分成假手术(Sham)组、心肌缺血再灌注模型(IR)组、红花黄色素预处理(HP,30 mg·kg~(-1))组、黄芪总皂苷预处理(AP,180 mg·kg~(-1))组和红芪复方预处理(HQP,红花黄色素30 mg·kg~(-1)+黄芪皂苷180 mg·kg~(-1))组(均n=10)。结扎大鼠左冠状动脉前降支复制心肌缺血再灌注模型,采用多道生理记录仪记录血流动力学指标,用放免法检测血清内皮素(ET)、6-酮前列腺素F1α(6-k-PGF1α)、血栓素B2(TXB2),酶联免疫法检测血清心肌肌钙蛋白I(cTnI)和心肌白细胞介素(IL)-6、肿瘤坏死因子α(TNF-α)含量,用化学比色法检测心肌丙二醛(MDA)含量,用紫外分光光度法检测心肌锰超氧化物歧化酶(Mn-SOD)活性。结果与IR组相比,HP组、AP组和HQP组左室收缩压(LVSP)、+dp/dtmax值在缺血期和再灌注期后均上升(P<0.05),HQP组显著高于HP组和AP组(P<0.05);与IR组相比,HP组、HQP组血清ET显著降低(P<0.05),HQP组TXB2显著降低(P<0.05),HQP组显著低于HP组和AP组(P<0.05),HQP组6-k-PGF_(1α)/TXB_2显著增高(P<0.05);与IR组相比,HP组、AP组和HQP组心肌中Mn-SOD活性也显著升高,MDA含量显著减少(P<0.05),心肌中IL-6和TNF-α含量显著降低(P<0.05),血清cTnI显著减少(P<0.05),HQP组比HP组和AP组改变更为显著(P<0.05)。结论红芪复方预处理可增强心肌抗氧化能力、改善心肌组织微循环、降低炎症反应、维持心肌泵血功能,对缺血再灌注心肌发挥保护作用,并且红花黄色素与黄芪总皂苷配伍的效果优于单味药。
Objective To investigate the protective effect of Radix Astragali compound preconditioning on myocardial ischemia-reperfusion injury in rats. Methods Fifty healthy male Sprague-Dawley rats were randomly divided into four groups: sham group, IR group, HP (30 mg · kg -1) group, Astragalus total saponin pretreatment (AP, 180 mg · kg -1) and HQP (HQP, safflower yellow pigment 30 mg · kg -1 + astragaloside 180 mg · kg ~ (-1) -1)) group (all n = 10). The left anterior descending coronary artery of rats was ligated and the models of myocardial ischemia-reperfusion were duplicated. The hemodynamic parameters were recorded by multi-channel physiological recorder. The levels of serum endothelin (ET) and 6-ketoprostaglandin F1α (6- The levels of serum cardiac troponin I (cTnI), myocardial interleukin (IL) -6 and tumor necrosis factor-α (TNF-α) were determined by enzyme-linked immunosorbent assay The content of malondialdehyde (MDA) in myocardium was detected by colorimetric method. The activity of Mn-SOD in myocardium was detected by UV spectrophotometry. Results Compared with IR group, LVSP and + dp / dtmax increased in both HP group, AP group and HQP group after ischemia and reperfusion (P <0.05), and were significantly higher in HQP group than those in IR group HP group and AP group (P <0.05). Compared with IR group, the level of serum ET in HP group and HQP group was significantly lower (P <0.05), TXB2 in HQP group was significantly lower (P <0.05) Compared with IR group, the activity of Mn-SOD in HP group, AP group and HQP group was significantly higher than that of AP group (P <0.05), HQP group (P <0.05) (P <0.05). The content of IL-6 and TNF-α in myocardium decreased significantly (P <0.05) and the level of cTnI in serum decreased significantly (P <0.05). Compared with HP group and AP group The change was more significant (P <0.05). Conclusions Radix Astragali compound pretreatment can enhance myocardial antioxidant capacity, improve myocardial microcirculation, reduce inflammatory reaction, maintain myocardial pump function and exert protective effect on myocardial ischemia-reperfusion injury, and safflower yellow and saponin total saponin compatibility The effect is better than single medicine.