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目的 探讨胎盘组织波形蛋白、Ⅳ型胶原蛋白和纤连蛋白的表达与妊娠高血压综合征(妊高征 )发病的关系。方法 采用免疫组化过氧化物酶 抗过氧化物酶 (PAP)法 ,检测 19例妊高征患者 (妊高征组 )胎盘组织波形蛋白、Ⅳ型胶原蛋白和纤连蛋白的表达情况 ,并与同孕周的正常孕妇15例 (对照组 )进行比较。结果 (1)中、重度妊高征患者胎盘组织波形蛋白阳性反应平均灰度值为30± 7,明显低于对照组的 4 2± 6和轻度妊高征患者的 4 2± 9,差异有极显著性 (P <0 0 1) ;(2 )中、重度妊高征患者胎盘组织Ⅳ型胶原蛋白阳性反应平均灰度值为 70± 6 ,较对照组的 2 9± 4和轻度妊高征患者的 30± 5明显增强 ,差异也有极显著性 (P <0 0 0 1) ;(3)中、重度妊高征患者胎盘组织纤连蛋白阳性反应平均灰度值为 37± 4 ,较对照组的 31± 4和轻度妊高征患者的 32± 6增强 ,差异有显著性 (P <0 0 5 )。结论 胎盘组织波形蛋白、Ⅳ型胶原蛋白和纤连蛋白表达异常 ,可能与妊高征的发病有密切关系 ,并且表达异常的程度随着病情加剧而明显
Objective To investigate the relationship between the expression of placental vimentin, type Ⅳ collagen and fibronectin and the pathogenesis of pregnancy induced hypertension (PIH). Methods The expression of vimentin, type Ⅳ collagen and fibronectin in 19 patients with PIH (placenta previa) was detected by immunohistochemical method of peroxidase-peroxidase (PAP) With the same gestational weeks in 15 cases of normal pregnant women (control group) were compared. Results (1), the average gray value of placental protein positive reaction in patients with severe PIH was 30 ± 7, significantly lower than 42 ± 6 in the control group and 42 ± 9 in patients with mild PIH (P <0.01). (2) The average gray value of type IV collagen in placenta of patients with moderate and severe PIH was 70 ± 6, which was 29 ± 4 and mild compared with that of control group (3) The average gray value of placental fibronectin positive reaction in patients with moderate and severe pregnancy-induced hypertension was 37 ± 4 , Which was significantly higher than that of control group (31 ± 4) and mild pregnancy-induced hypertension (32 ± 6) (P <0.05). Conclusions The abnormal expression of placental vimentin, type Ⅳ collagen and fibronectin may be closely related to the pathogenesis of PIH, and the degree of abnormal expression is obvious with the aggravation of the disease