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本实验应用重组逆转录病毒(LNCX)为载体，将潮霉素B磷酸转移酶基因与Ⅰ型单纯疱疹病毒胸苷激酶基因的融合基因(HyTK)导入大鼠颈动脉壁细胞中，配合应用无环鸟苷(ACV），观察对内皮细胞(EC)剥脱后血管平滑肌细胞(SMC)增生的抑制作用。1 材料与方法1.1 动物：国产SD大鼠，雄性300
In this study, the recombinant retrovirus (LNCX) was used as a vector to transduce the HyTK gene of hygromycin B phosphotransferase gene and type I herpes simplex virus thymidine kinase gene into rat carotid artery wall cells, Cyclic guanosine (ACV) was used to observe the inhibitory effect on proliferation of vascular smooth muscle cells (SMC) after exfoliation of endothelial cells (ECs). 1 Materials and Methods 1.1 Animals: Domestic SD rats, male 300