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目的:利用LNCaP前列腺癌裸鼠模型,观察PSA特异性树突状细胞(dendritic cell,DC)瘤苗(PSA-DC)体外诱导的CTL体内过继输入的抗肿瘤效果。方法:采用裸鼠皮下接种LNCaP细胞的方法建立LNCaP前列腺癌荷瘤裸鼠模型;应用前期制备好的Non-DC、Ova-DC、Lys-DC及PSA-DC瘤苗体外诱导抗原特异性CTL细胞,并在LNCaP细胞接种第15天模型制备成功时首次将体外诱导的抗原特异性CTL细胞经尾静脉过继输入小鼠体内,7d后重复输入1次。以初次治疗后50d为终止点,观察肿瘤生长情况;观察各组荷瘤裸鼠的存活情况。结果:过继输入治疗后第30、50天,Non-DC、Ova-DC、Lys-DC及PSA-DC组瘤体明显大于肿瘤细胞接种第15天(P<0.01);Lys-DC、PSA-DC组瘤体明显小于Non-DC、Ova-DC组(P<0.01)。在观察期间内,Lys-DC、PSA-DC组裸鼠生存时间明显长于Non-DC、Ova-DC组(P<0.01)。结论:前列腺癌瘤苗PSA-DC体外诱导的PSA特异性CTL细胞过继输入可抑制裸鼠体内LNCaP肿瘤生长,提高裸鼠生存时间。
OBJECTIVE: To study the anti-tumor effect of adoptive transfer of CTL induced by PSA-DC in vitro in LNCaP prostate cancer nude mice model. Methods: The nude mouse model of LNCaP prostate cancer was established by subcutaneous inoculation of LNCaP cells in nude mice. Antigen-specific CTL cells were induced in vitro using non-DC, Ova-DC, Lys-DC and PSA- , And the first time in the LNCaP cells on the 15th day when the model was successfully prepared, the in vitro induced antigen-specific CTL cells were transfused into the mice via the tail vein, and once again after 7 days. The first 50 days after the treatment as the termination point, observed tumor growth; observed nude mice in each group survival. Results: The tumors in Non-DC, Ova-DC, Lys-DC and PSA-DC groups were significantly larger than that in tumor cells on the 15th and the 50th day after treatment (P <0.01) The tumor volume of DC group was significantly lower than that of Non-DC and Ova-DC groups (P <0.01). During the observation period, the survival time of Lys-DC and PSA-DC groups was significantly longer than that of Non-DC and Ova-DC groups (P <0.01). Conclusion: Prostate cancer vaccine PSA-DC in vitro induced by PSA-specific CTL cells adoptive input can inhibit LNCaP tumor growth in nude mice and improve survival time in nude mice.