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大鼠实验模型需血时,通常采用尾静脉、断头取血等方法。不能对整体模型反复采血。我们用颈外静脉保留性插管的方法不仅能反复、多次收集血液标本、静脉给药、进行自身对照,并为实验研究提供了进行动态观察的条件。导管的制备:取内径约0.6mm的聚乙烯管(PE50)10.5cm长,在距一端1cm处均匀加热,使其形成一个圆珠样膨大。另取一内径0.8mm的硅胶软管2.5cm,将其套在前管膨大近端,在
Rat experimental model blood, usually tail vein, decapitated blood and other methods. Can not take blood repeatedly on the whole model. We use cannulated external jugular vein catheterization method can not only repeatedly, repeatedly collected blood samples, intravenous administration, self-control, and provide experimental conditions for dynamic observation. Preparation of the catheter: A polyethylene tube (PE50) having an inner diameter of about 0.6 mm was taken for a length of 10.5 cm and uniformly heated at a distance of 1 cm from one end to form a ball-like enlargement. Another take an inner diameter of 0.8mm silica gel hose 2.5cm, set it in the front tube inflated proximal, at