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【目的】获得能够在高盐环境下脱色偶氮染料的高效脱色菌群,应用于印染废水的生物处理。【方法】采用在5%盐度培养基富集的方法,从印染废水的活性污泥中富集能够在5%盐度下脱色酸性大红GR的嗜盐混合菌群,利用高通量测序方法研究其群落结构,利用静置培养的方法研究其脱色性能。【结果】该菌群可以在5%盐度、静置培养下15 h内将100 mg/L的酸性大红GR几乎完全脱色,主要由Halomonas、Salinicoccus、Nitratireductor和Aequorivita等4个属组成,Halomonas是主要的脱色菌。高浓度的Na NO_3、Na_2SO_4和Na Cl抑制菌群的脱色,其中Na NO_3抑制作用最强。该菌群的最佳脱色条件是在p H 7.0、盐度5%、30°C脱色效果最好,可脱色直接耐黑G和分散深蓝S-3BG等偶氮染料,并且具有连续脱色的能力。【结论】嗜盐菌群在处理偶氮染料废水中具有良好的应用价值。
【Objective】 The objective of this study was to obtain a highly efficient decolorizing bacterial population capable of decolorizing azo dyes in high salt environment and to apply it to the biological treatment of printing and dyeing wastewater. 【Method】 The halophilic mixed bacteria population that can degrade acid red GR at 5% salinity was enriched from the activated sludge of printing and dyeing wastewater by 5% salinity medium enrichment method. High-throughput sequencing Study their community structure, the use of static culture method to study its decolorization performance. 【Result】 The strain could almost completely decolorize 100 mg / L Acid Scarlet GR in 5% salinity within 15 h under static culture. It mainly consisted of 4 genera, Halomonas, Salinicoccus, Nitratireductor and Aequorivita. Halomonas was The main bleaching bacteria. High concentrations of NaNO_3, Na_2SO_4 and NaCl inhibited the decolorization of the flora, of which NaNO_3 had the strongest inhibitory effect. The optimal decolorization conditions of this strain were the best decolorization effect at p H 7.0, 5% salinity and 30 ° C, azo dyes such as direct-resistant black G and disperse dark blue S-3BG, with continuous decolorization . 【Conclusion】 Halophilic bacteria have a good application value in the treatment of azo dye wastewater.