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用正交法考察了各因素对浸取纯化三七总皂苷的影响,确立了离子交换纤维纯化三七总皂苷的最佳工艺.结果表明:浸取三七总皂苷的最佳条件为温度65℃,时间2 h,浸提剂体积20mL/g(三七粉),浸提次数3次,浸提率7.68%;在质量浓度为1.152 mg.mL-1条件下,强碱阴离子交换纤维静态吸附三七总皂苷的最佳条件是温度65℃,pH=8,皂苷体积250 mL/g(纤维),吸附率为91.02%;吸附在强碱阴离子交换纤维上的三七总皂苷的静态解吸的最佳条件是pH=1,温度70℃,解吸剂质量分数60%和解吸剂体积900 mL/g(饱和纤维),解吸率92.21%.上述结果表明用强碱阴离子交换纤维提取纯化三七总皂苷是可行的.
The effects of various factors on the total saponins extracted from Panax notoginseng by orthogonal test were investigated by orthogonal test.The optimum conditions for the purification of Panax notoginseng saponins by ion exchange fiber were established.The results showed that the optimal conditions for the total saponins notoginseng saponification were temperature 65 ℃ for 2 h, the volume of extractant was 20 mL / g (notoginseng powder), the extraction time was 3 times and the extraction rate was 7.68%. Under the condition of mass concentration of 1.152 mg.mL-1, the strong base anion exchange fiber was static The optimum conditions for the adsorption of Panax notoginseng saponins were as follows: temperature 65 ℃, pH = 8, saponin volume 250 mL / g (fiber), the adsorption rate was 91.02%; static desorption of Panax notoginseng saponins adsorbed on strongly basic anion exchange fibers The optimum conditions were as follows: pH = 1, temperature 70 ℃, desorbent mass fraction 60% and desorbent volume 900 mL / g (saturated fiber), desorption rate 92.21% Total saponins are feasible.