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检测BCR-ABL-pIRES-SEA重组基因疫苗在小鼠肌肉中的表达。用已构建BCR-ABL-pIRES-SEA重组质粒免疫BALB/c小鼠一侧后肢骨骼肌,每两周一次,每次注射200μg,第七周取材用RT-PCR及免疫组织化学染色法检测目的基因在注射部位肌肉中转录和表达。结果发现:实验小鼠骨骼肌内检测到BCR-ABL和金黄色葡萄球菌肠毒素A(SEA)基因的转录,以及BCR-ABL蛋白的表达,证明所构建的BCR-ABL-pIRES-SEA重组质粒在小鼠体内可以有效地表达基因产物。
The expression of BCR-ABL-pIRES-SEA recombinant gene vaccine in mouse muscle was detected. BALB / c mice were immunized with recombinant plasmid of BCR-ABL-pIRES-SEA on the hindlimb skeletal muscle on one side every two weeks for 200μg each time. The seventh week was used RT-PCR and immunohistochemistry to detect the purpose Genes are transcribed and expressed in the muscle of the injection site. The results showed that the transcription of BCR-ABL and Staphylococcus aureus enterotoxin A (SEA) gene and the expression of BCR-ABL protein were detected in the skeletal muscle of experimental mice, demonstrating that the BCR-ABL-pIRES-SEA recombinant plasmid Gene products can be efficiently expressed in mice.