论文部分内容阅读
本发明者们在反复研究各种生药提取物药理作用过程中,发现生药的提取物具有优良的抑制血小板凝集作用。现将实验方法及结果介绍如下。样品液的制备将100g干燥了的生药细切粉末用50%乙醇300ml加热回流3小时后,提取、过滤、浓缩、干燥该提取液,再以10mg/ml的比率,溶解在生理盐水中,作为样品液。或者把悬浮的上清部分作为样品溶液。凝集剂:将二磷酸腺甙(ADP、钠盐)的生理盐水溶液(调制成0.05μΜ的最高浓度)作为ADP溶液。另外,在胶原(不溶性)中加生理盐水,用乳钵进行匀化,以1000rpm的速度,离心分离10分钟,把所获得的上清部分作为胶原液。血小板的制备
The inventors of the present invention have repeatedly studied the pharmacological action of various crude drug extracts and found that the crude drug extract has an excellent effect of inhibiting platelet aggregation. The experimental methods and results will now be described as follows. Preparation of sample solution After 100 g of the dried fine powder of crude drug was heated and refluxed with 300 ml of 50% ethanol for 3 hours, the extract was extracted, filtered, concentrated and dried, and dissolved in physiological saline at a ratio of 10 mg/ml. Sample solution. Alternatively, the suspended supernatant can be used as a sample solution. Agglutinating agent: A physiological saline solution of adenosine diphosphate (ADP, sodium salt) (prepared to a maximum concentration of 0.05 μM) was used as an ADP solution. In addition, physiological saline was added to collagen (insoluble), homogenized in a mortar, centrifuged at 1000 rpm for 10 minutes, and the obtained supernatant was used as a collagen solution. Platelet preparation