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用 Merrifield 方法合成大肽(含有30个以上氨基酸残基)时,由于缺陷肽的积累,使最终产物的分离纯化十分困难,固相片段缩合往往可以减少这些麻烦.后一方法的主要问题是能否快速有效地合成保护肽段.固相合成保护肽的关键在于设计一个有特定结构的载体,以使合成的肽从树脂上断裂下来时,不影响各类保护基和不产生(或少产生)其他反应.关于这方面的研究有很多报道,但尚未能得到满意的结果.
When the large peptide (containing more than 30 amino acid residues) is synthesized by the Merrifield method, separation and purification of the final product is very difficult due to the accumulation of defective peptides, and solid phase fragment condensation tends to reduce these troubles. The main problem of the latter method is that energy The key to the synthesis of the protective peptide in the solid state reaction is to design a carrier with a specific structure so that the synthesized peptide breaks down from the resin without affecting various kinds of protecting groups and not generating ) Other reactions There have been many reports of research in this area, but not yet satisfactory results.