论文部分内容阅读
目的分析北京市2010-2015年分离的259株宋内志贺菌脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分型及耐药特征。方法收集2010-2015年分离到的宋内志贺菌259株,经生化及血清型鉴定后,应用PFGE分型并用最小抑菌浓度法(minimal inhibition concentration,MIC)进行12种药物的耐药检测。结果 259株宋内志贺菌经PFGE图谱聚类分析后,发现宋内志贺菌有A、B两个优势簇,2010-2015年A簇菌株所占构成比逐年增高;耐药结果显示257株为耐药菌株,其中245株为多耐药菌株,占所有耐药菌株的95.33%。对头孢曲松(CRO)的耐药性有明显升高。结论北京地区近年宋内志贺菌有较高的同源性,A簇菌株已经成为目前北京地区宋内志贺菌的优势簇。对三代头孢类药物CRO等耐药性上升可能与临床上大量应用三代头孢药物以及A簇菌株增多有关,多耐药谱的情况也更加复杂,提示应合理使用抗生素。
Objective To analyze the typing and drug resistance characteristics of 259 strains of Shigella sonnei isolated from Beijing in 2010-2015 using pulsed field gel electrophoresis (PFGE). Methods 259 strains of Shigella sonnei isolated from 2010 to 2015 were collected. After biochemical and serological identification, PFGE typing and minimal inhibit concentration (MIC) were used to detect the resistance of 12 kinds of drugs . Results After 259 strains of Shigella sonnei were clustered by PFGE analysis, Shigella sonnei had two dominant clusters, A and B, and the proportion of cluster A strains in 2010-2015 increased year by year. The resistance results showed that 257 Among them, 245 strains were multidrug-resistant strains, accounting for 95.33% of all resistant strains. Resistance to ceftriaxone (CRO) was significantly increased. Conclusion In recent years, Shigella has high homology in Beijing and A cluster has become the dominant cluster of Shigella sonnei in Beijing. The third generation of cephalosporins CRO and other drug resistance may be increased with the clinical application of a large number of third-generation cephalosporins and A cluster of strains related to multi-drug resistance spectrum is more complex, suggesting that rational use of antibiotics.