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应用增殖细胞核抗原（ＰＣＮＡ）和Ｐ１８５单克隆抗体，通过免疫组织化学方法检测５例正常肾组织和４５例肾癌组织中Ｃ－ｅｒｂＢ－２癌基因产物Ｐ１８５蛋白和ＰＣＮＡ的表达状况。结果：５例正常肾组织中未发现Ｐ１８５蛋白和ＰＣＮＡ阳性表达；４５例肾癌中Ｐ１８５蛋白和ＰＣＮＡ阳性表达率分别为８４．４％和４４．４％。表明阳性表达的Ｐ１８５蛋白和ＰＣＮＡ分别定位于肿瘤的细胞膜上和细胞核内；Ｐ１８５蛋白和ＰＣＮＡ阳性表达率与肾癌的病理分级、临床分期和患者术后生存期相关。提示Ｐ１８５蛋白和ＰＣＮＡ阳性表达在肾癌发生和发展中起着重要作用，可作为评价肾癌预后的新参数。
The expression of C-erbB-2 oncogene P185 protein and PCNA in 5 normal renal tissues and 45 renal cell carcinoma tissues were detected by immunohistochemistry with PCNA and P185 monoclonal antibodies. Results: No positive expression of P185 protein and PCNA was found in 5 cases of normal renal tissues. The positive rates of P185 protein and PCNA in 45 cases of renal cell carcinoma were 84.4% and 44.4%, respectively. The positive expression of P185 protein and PCNA were located on the cell membrane and the nucleus of the tumor, respectively. The positive rates of P185 protein and PCNA were correlated with the pathological grade, clinical stage and survival time of patients with renal cell carcinoma. It is suggested that the positive expression of P185 protein and PCNA plays an important role in the occurrence and development of renal cell carcinoma and may be used as a new parameter to evaluate the prognosis of renal cell carcinoma.