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bZIP基因在植物响应非生物胁迫过程中发挥重要的调控作用。我们从大豆中克隆到一个bZIP基因GmbZ IP60(Genebank Accession No:DQ787038),并成功转化到拟南芥中。本研究在此基础上,利用qRT-PCR和GUS组织化学染色法研究了髙温(37℃)、低温(4℃)、干旱(PEG2000)、NaCl(200 mmol·L~(-1))和ABA(100 mmol·L~(-1))处理对GmbZIP60表达的影响。结果表明:髙温(37℃)、低温(4℃)、干旱(PEG2000)和NaCl(100 mmol·L~(-1))胁迫处理下,GmbZIP60表达量显著降低,分别变为对照组的0.07倍、0.25倍、0.36倍和0.13倍,而ABA处理对GmbZIP60表达量影响不显著,GUS染色结果与荧光定量PCR结果一致。研究认为,GmbZIP60可能以负调控的方式参与大豆抗高盐、干旱、髙温、低温等非生物胁迫的应答反应。本研究为进一步鉴定和克隆大豆逆境应答关键基因,揭示大豆抗逆分子机制和大豆抗逆性基因工程改良提供了有益借鉴。
The bZIP gene plays an important regulatory role in plant response to abiotic stress. We cloned a bZIP gene, GmbZ IP60 (Genebank Accession No: DQ787038) from soybean and successfully transformed it into Arabidopsis thaliana. On the basis of this study, qRT-PCR and GUS histochemical staining were used to study the effects of temperature, Effect of ABA (100 mmol·L -1) on the expression of GmbZIP60. The results showed that the expression of GmbZIP60 was significantly reduced under the stress of 37 ℃, 4 ℃, PEG2000 and NaCl (100 mmol·L -1) Times, 0.25 times, 0.36 times and 0.13 times, respectively. However, ABA treatment had no significant effect on the expression of GmbZIP60. The results of GUS staining and fluorescence quantitative PCR were consistent. The research suggests that GmbZIP60 may participate negatively in the response of soybean to abiotic stresses such as high salt, drought, cold and low temperature. This study provides a useful reference for further identification and cloning of key genes in response to soybean stress, revealing molecular mechanisms of soybean resistance and genetic improvement of soybean stress resistance.