Expression and purification of soluble fusion protein of Toxoplasma gondii GRA6 in E. coli.

• 作 者: 朱翔
• 机 构: Suzhou 5th People's Hosp Suzhou 215006,
• 出 处: China Medical Abstracts(Internal Medicine)
• 发表日期: 2006年04期
• 关键词: Toxoplasma purification bacterium transformed Codon isopropyl plasmid infective

Objective To high express and purify toxoplasma gondii antigen GRA6 in E. coli which can be used to develop the genetic engineering diagnostic reagents. Methods The recombinant plasmid of pGEX-GRA6 was transformed to a bacterium BL21-Codon Plus (DE3)-RP and the recombinant product was expressed under the inducement of isopropyl-beta-D-thiogalactosidase(IPTG).

Objective To high express and purify toxoplasma gondii antigen GRA6 in E. coli which can be used to develop the genetic engineering diagnostic reagents. Methods The recombinant plasmid of pGEX-GRA6 was transformed to a bacterium BL21-Codon Plus (DE3) -RP and the recombinant product was expressed under the inducement of isopropyl-beta-D-thiogalactosidase (IPTG).