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对杂交瘤细胞产生的抗体而言,重组抗体代表第二代抗体,它是在体外构建并在转染细胞中表达的免疫球蛋白(Ig)基因的产物。最近,依靠基因工程技术和基因转移方法相结合,已有可能构建具有预定特异性的Ig基因,并使其在骨髓瘤细胞中表达。实验设计重组抗体的表达是在穿梭载体上构建近C基因端的、重排V基因,并将这些基因转染骨髓瘤细胞,由此获得分泌抗体。重组Ig基因的类型骨髓瘤或杂交瘤的VH基因与各种同型C_H基因组合,并完成种内同型转换,可产生重组抗体。种间V-C的组合产生嵌合抗体。通常以小鼠V区与人C区结合产生一种已知特异性的近似人类的抗
For antibodies produced by hybridoma cells, the recombinant antibody represents a second generation antibody, which is the product of an immunoglobulin (Ig) gene that is constructed in vitro and expressed in transfected cells. Recently, relying on a combination of genetic engineering techniques and gene transfer methods, it has been possible to construct Ig genes with a predetermined specificity and to allow them to be expressed in myeloma cells. Experimental Design Recombinant antibodies are expressed by constructing proximal C-terminal, rearranged V genes on a shuttle vector and transfecting these genes into myeloma cells, thereby obtaining secreted antibodies. The type of recombinant Ig gene The VH gene of myeloma or hybridoma is combined with various homo-C_H genes and an in-species homologous switch is made to produce a recombinant antibody. The combination of interspecies V-C results in a chimeric antibody. Binding to mouse C region and human C region usually produces a known and specific approximate human resistance